Question

The PCR protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, Wyoming. This organism contains a heat-stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been heated to 95 degrees C. Why would such a heat-stable polymerase be beneficial in PCR

Answer 1

Answer:

The correct answer is - Each cycle includes a "hot" denaturation phase (95C), which separates the hydrogen bonds that hold the strands of the template DNA together.

Explanation:

PCR is a polymerase chain reaction created by karry Mullis which is utilized to amplify a copy of DNA segments or a single copy of DNA or gene. The following are the steps involved in PCR reaction;

Denaturation: During this progression, the two-fold strand of DNA gets separated. The temperature required for this progression is 94 — 98 C.  

Annealing: during this progression, Template DNA and DNA primers get attached. The temperature required for this progression is 55 — 70 C  

Extension: During this progression, the polymerase includes the bases that are reciprocal to the template DNA strand. The temperature required for this progression is 65 — 72 C.  

At such high temperatures, DNA polymerase gets denatured. As it very well may be found in the above strides for the isolation of the DNA strand high temperature of 94 — 98 C Is utilized. Taq polymerase can withstand such high temperatures.