Answer:
The correct option is : cells containing empty plasmid vectors
Explanation:
The blue-white screen is a technique which involves the rapid identification of the recombinant bacteria in a vector-based molecular cloning experiment. In this method, a DNA ligated vector is inserted in a host cell which is viable for transformation and grown in presence of X-gal.
The cells that are transformed with the vectors having the recombinant DNA produce the white colonies. Whereas, the cells transformed with empty vector i.e. the non-recombinant plasmids, produce the blue colonies.
Answer:
2 molecules of ATP and 2 molecules of NADH
Explanation:
Glycolysis is the first step of cellular respiration (break down of glucose to extract energy) which occurs in the cytoplasm. Glycolysis is a pathway common to all living organisms- prokaryotes and eukaryotes, as it does not require oxygen to occur.
Glycolysis occurs in two major phases (ten steps) requiring 10 enzymes catalyzing each step; the energy-requiring phase and the energy-requiring phase.
In the energy-requiring phase, the starting molecule (glucose) gets rearranged in a series of chemical reactions, and two phosphate groups gets attached to it producing fructose-1,6-bisphosphate which is unstable, This modified sugar then splits in half due to its instability to form two different but inter-convertible phosphate-bearing three-carbon sugars (Dihydroxyacetonephosphate, DHAP and Glyceraldehyde-3-phosphate, G3P). Because the phosphates used in these steps come from 2 ATP molecules, 2 ATP molecules get used up in this phase
All the DHAP molecules get converted to G-3-P in order to enter the next phase.
In the energy-recovering phase, the 3-carbon sugar (G3P) is converted into another three-carbon molecule called pyruvate, through a series of reactions. In these reactions, two ATP and 1 NADH molecules are made. This recovery phase occurs twice (one for each of the two isomeric three-carbon sugars, DHAP and G3P). Hence, a total of 4 ATP and 2 NADH molecules are produced in this phase.
Overall, Glycolysis converts one glucose (six-carbon) molecule to two pyruvate (three-carbon) molecules and a net release of 2 ATP molecules (4 overall - 2 used) and 2 NADH molecules.
Answer:
Tetraploid wheat evolved by allopolyploidization and subsequent diploid-like behavior due to cytological diploidization
Explanation:
Durum wheat (<em>Triticum durum</em>) or pasta wheat, is a tetraploid wheat species that has 28 chromosomes, i.e., seven pairs in each genome (2n = 4x = 28). Durum wheat was domesticated from wild emmer wheat, which originated by hybridization of two diploid wheat species with 14 chromosomes: <em>Triticum monococcum</em> (genome AA) and one wild progenitor (genome BB). <em>Triticum durum </em>is a typical example of evolution by hybridization and polyploidization, where the resultant tetraploid species has two complete sets of chromosomes. Allopolyploidization is one of the most common types of plant speciation. During meiosis, 28 chromosomes form 14 homologous chromosome pairs, because homologous chromosomes have developed 'restriction of pairing' (i.e., cytological diploidization). The restriction of pairing to fully homologous chromosomes ensures a correct meiotic behavior, which otherwise would be altered due to the high level of homology that still exists among chromosomes from different wheat progenitors.
atom, molecule, cells, tissues, organ, organ system, organism
Answer:
D
Explanation:
Prokaryotic are simple cells and do not have nucleus.