DNA isolation is a process of purification of DNA from sample using a combination of physical and chemical method while on the other hand, Polymerase chain reaction (PCR<span>) is a technique used in molecular biology to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.</span>
For any enthalpy change in a chemical reaction, (exothermic/endothermic), the activation energy must be reached so as to break the strong chemical bonds between the reactant molecules.
This varieation can be justified because since they had different variations they probably had different niches which led them to evolve differently,this will help because the two species will have no competition