Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Answer:
Hope this helps!
Explanation:
"When your body is injured in some way or something else is wrong, your nerves (cells that help your body send and receive information) send millions of messages to your brain about what's going on. Your brain then makes you feel pain." -Why Do I Have Pain? (for Kids) - Nemours KidsHealth
I didn't write that, but I gave credit to whoever did.
Both "depends upon the binding of that substance to a specific site on the membrane protein" and "depends upon movement of proteins from one side of the membrane to the other" are correct.
The saturation effect prevents statement 2 from being true.
Answer: m <- that's the answer
Explanation: