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matrenka [14]
3 years ago
10

Cultures glowing under UV light annotate transformation of the original bacteria with the pGLO plasmid. Bacteria without the pGL

O plasmid will neither glow under UV light nor be ampicillin resistant. If this is true, how can we have healthy, non-glowing, non-ampicillin resistant E. coli colonies growing on the LB/Amp/Ara plate
Chemistry
1 answer:
victus00 [196]3 years ago
7 0

Answer: Contamination

Explanation: This is a known contrainst when performing transformation in the lab. Normally the Pglo plasmid asides glowing under UV, possess a Amp gene that codes for resistance to the antibiotics Ampicillin. Transform cells will survive while untransformed cells will normally die. If untransformed (non glowing, non Ampicillin resistant) cells thrive in the medium, it is definitely a case of contamination. Start again but this time, disinfect appropriately bad be cautious of any potential contamination.

You might be interested in
What type of information can one obtain by taking a mass spectrum of an organic molecule like dodecane?
Furkat [3]
Mass spectrum of Dodecane will give following information.

1 ) Molecular Peak or Parent Peak:
                                                       The Parent peak will appear at m/z = 170. The intensity of this peak will be very weak.

2) Fragments:
                      Usually the fragments of such long chain alkanes appear with spacing of 14 amu, Hence, the peaks in dodecane will be as follow,

         170 - 156 - 142 - 128 - 114 - 100 - 86 - 72 - 58 - 44 - 30 - 16

3) Base Peak:
                     Most probably the Base peak will appear at m/z = 57. This peak is due to the formation of tertiary butyl cation as the intensity mainly depends upon the stability of cation. So this cation might form due to rearrangment giving the intensity of 100%.
8 0
3 years ago
Final Temperature in Heating Applesauce. A mixture of 454 kg of applesauce at 10°C is heated in a heat exchanger by adding 12130
jok3333 [9.3K]

Answer:

T_f = 76.46°C

Explanation:

Given data:

Mass of mixture = 454 kg

Initial temperature is 10°C

Heat added is Q = 121300 kJ

Heat capacity (Applesuace) at 32.8°C is 4.02kJ/kg K

From heat equation we have

Q = mCp(T_f -T_i)

\frac{Q}{mCp} = (T_f -T_i)

T_f = T_i + \frac{Q}{mCp}

Putting all value to get required final temperature value

T_f = \frac{121300}{454\times 4.02} + 10

T_f = 76.46°C

7 0
3 years ago
Which set of numbers gives the correct possible values of I for n = 2?
Marysya12 [62]

Answer:

0,1

Explanation:

There are four quantum numbers:

Principal quantum number (n)

Azimuthal quantum number (l)

Magnetic quantum number (ml)

Spin quantum number (ms)

All these four quantum numbers gives complete information about an electron like its spin, shells, subshells and orbitals.

For example:

If n=2 than possible sets of quantum numbers are:

Azimuthal quantum number (l)

The azimuthal quantum number describe the shape of orbitals. Its value for s, p, d, f... are 0, 1, 2, 3. l= n-1

(n-1)

2-1 = 1

For n=2 there are two subshell s and p.

s = 0

p= 1

Magnetic quantum number (ml)

It describe the orientation of orbitals. Its values are -l to +l. For l=1  the ml will be  -1 0 +1

Spin quantum number (ms)

The spin quantum  number tells the spin of electron either its clock wise (+1/2) or anti clock wise (-1/2).

If the electron is added in full empty orbital its spin will be +1/2 because it occupy full empty. If electron is already present and another electron is added then its spin will be -1/2.

4 0
3 years ago
A 50.0 mL solution of 0.141 M KOH is titrated with 0.282 M HCl . Calculate the pH of the solution after the addition of each of
Kobotan [32]

Answer:

pH =1 2.84

Explanation:

First we have to start with the <u>reaction</u> between HCl and KOH:

HCl~+~KOH->~H_2O~+~KCl

Now <u>for example, we can use a volume of 10 mL of HCl</u>. So, we can calculate the moles using the <u>molarity equation</u>:

M=\frac{mol}{L}

We know that 10mL=0.01L and we have the concentration of the HCl 0.282M, when we plug the values into the equation we got:

0.282M=\frac{mol}{0.01L}

mol=0.282*0.01

mol=0.00282

We can do the same for the KOH values (50mL=0.05L and 0.141M).

0.141M=\frac{mol}{0.05L}

mol=0.141*0.05

mol=0.00705

So, we have so far <u>0.00282 mol of HCl</u> and <u>0.00705 mol of KOH</u>. If we check the reaction we have a <u>molar ratio 1:1</u>, therefore if we have 0.00282 mol of HCl we will need 0.00282 mol of KOH, so we will have an <u>excess of KOH</u>. This excess can be calculated if we <u>substract</u> the amount of moles:

0.00705-0.00282=0.00423mol~of~KOH

Now, if we want to calculate the pH value we will need a <u>concentration</u>, in this case KOH is in excess, so we have to calculate the <u>concentration of KOH</u>. For this, we already have the moles of KOH that remains left, now we need the <u>total volume</u>:

Total~volume=50mL+10mL=60mL

60mL=0.06L

Now we can calculate the concentration:

M=\frac{0.00423mol}{0.06L}

M=0.0705

Now, we can <u>calculate the pOH</u> (to calculate the pH), so:

pOH=-Log(0.0705)

pOH=1.15

Now we can <u>calculate the pH value</u>:

14=~pH~+~pOH

pH=14-1.15=12.84

8 0
3 years ago
If a certain gas occupies a volume of 10. L when the applied pressure is 5.0 atm , find the pressure when the gas occupies a vol
lorasvet [3.4K]

Answer:

20L is the new volume

Explanation:

In this case, moles and T° from the gas remain constant. This is the formula we must apply, to solve this:

P₁ . V₁ = P₂ . V₂

5 atm . 10 L = P₂ . 2.5L

P₂ = (5 atm . 10 L) / 2.5L →20L

5 0
3 years ago
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