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matrenka [14]
2 years ago
10

Cultures glowing under UV light annotate transformation of the original bacteria with the pGLO plasmid. Bacteria without the pGL

O plasmid will neither glow under UV light nor be ampicillin resistant. If this is true, how can we have healthy, non-glowing, non-ampicillin resistant E. coli colonies growing on the LB/Amp/Ara plate
Chemistry
1 answer:
victus00 [196]2 years ago
7 0

Answer: Contamination

Explanation: This is a known contrainst when performing transformation in the lab. Normally the Pglo plasmid asides glowing under UV, possess a Amp gene that codes for resistance to the antibiotics Ampicillin. Transform cells will survive while untransformed cells will normally die. If untransformed (non glowing, non Ampicillin resistant) cells thrive in the medium, it is definitely a case of contamination. Start again but this time, disinfect appropriately bad be cautious of any potential contamination.

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What is the gram formula of na2co3
makkiz [27]

Answer:

106 gfm

Explanation:

element. number masses

Na. 2 23×2

C. 1. 12

O. 3. 16×3

then add

106 gfm

5 0
2 years ago
If there is a graduated cylinder containing 20mL of water and a small rock is gently placed inside it, and the water level insid
LekaFEV [45]

Answer:

5

Explanation:

just subtract 20-25 and 5 is left over which is how much the water when up with the rock.

8 0
3 years ago
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Nitric oxide (NO) can be formed from nitrogen, hydrogen and oxygen in two steps. In the first step, nitrogen and hydrogen react
son4ous [18]

Answer:

ΔH  = - 272 kJ

Explanation:

We are going to use the fact that Hess law allows us to calculate the enthalpy change of a reaction no matter if the reaction takes place in one step or in several steps. To do this problem we wll add two times the first step to second step as follows:

N2(g) +         3H2(g) →          2NH3(g)                 ΔH=−92.kJ  Multiplying by 2:      

2N2(g) +       6H2(g) →          4NH3(g)                        ΔH=− 184 kK

plus

4NH3(g) +     5O2(g) →          4NO(g) +6H2O(g)        ΔH=−905.kJ

__________________________________________________

2N2(g) +   6H2(g) + 5O2(g)→  4NO(g)  + 6H2O(g)      ΔH = (-184 +(-905 )) kJ

                                                                                     ΔH =    -1089 kJ

Notice how the intermediate NH3 cancels out.

As we can see this equation is for the formation of 4 mol NO, and we are asked to calculate the ΔH  for the formation  of one mol NO:

-1089 kJ/4 mol NO  x 1 mol NO =  -272 kJ (rounded to nearest kJ)

5 0
3 years ago
What is called when a paragraph studies data carefully
Zigmanuir [339]
Paraphrasing and summarizing
8 0
3 years ago
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A horizontal cylinder equipped with a frictionless piston contains 785 cm3 of steam at 400 K and 125 kPa pressure. A total of 83
guapka [62]

Answer:

a. 478.69 K

b. 939.43 cm^{3}

c. 19.30 J

d. 64.5J

Explanation:

From the question, we can identify the following;

V_{o} = 785cm^{3} = 0.000785 m^{3}

T_{o} = 400K

P_{o} = 125 Kpa =  125 000 Pa

Using the ideal gas equation,

PV = nRT

where R is the molar gas constant = 8.314 m^{3}⋅Pa⋅K^{-1}⋅mol^{-1}

Thus, n = PV/RT = (125000 × 0.000785)/(8.314 × 400) = 0.03 mol

a. Steam temperature in K

To calculate this, we use the constant pressure process;

q = nΔH

Where q is 83.8J according to the question

Thus;

83.8 = 0.03 × [34980 + 35.5T_{1} - (34980 + 35.5T_{o})]

83.8 = (0.03 × 35.5) (T_{1} - 400K)

83.8 = 1.065 (T_{1}  - 400)

78.69 = (T_{1}  - 400)

T_{1} = 400 + 78.69

T_{1}  = 478.69 K

b. Final cylinder volume

To calculate this, we make use of the Charles' law(Temperature and pressure are directly proportional)

V_{1}/T_{1} = V_{o}/T_{o}

V_{1}  =  V_{o}T_{1}/T_{o}

V_{1}   = (785 × 478.69)/400

V_{1}   = 939.43 cm^{3}

c. Work done by the system

Mathematically, the work done by the system is calculated as follows;

w = P(V_{1}- V_{o}) = 125 KPa ( 939.43 - 785) = 19.30 J

d. Change in internal energy of the steam in J

ΔU = q - w = 83.8 - 19.3 = 64.5J

6 0
3 years ago
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