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matrenka [14]
3 years ago
10

Cultures glowing under UV light annotate transformation of the original bacteria with the pGLO plasmid. Bacteria without the pGL

O plasmid will neither glow under UV light nor be ampicillin resistant. If this is true, how can we have healthy, non-glowing, non-ampicillin resistant E. coli colonies growing on the LB/Amp/Ara plate
Chemistry
1 answer:
victus00 [196]3 years ago
7 0

Answer: Contamination

Explanation: This is a known contrainst when performing transformation in the lab. Normally the Pglo plasmid asides glowing under UV, possess a Amp gene that codes for resistance to the antibiotics Ampicillin. Transform cells will survive while untransformed cells will normally die. If untransformed (non glowing, non Ampicillin resistant) cells thrive in the medium, it is definitely a case of contamination. Start again but this time, disinfect appropriately bad be cautious of any potential contamination.

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What is the pH of a solution that contains 0.003 M HBr?*<br> O 1.0<br> O 2.5<br> O 5.8<br> O 11.5
maxonik [38]

Answer:

pH = 2.5

Explanation:

Given data:

Concentration of HBr = 0.003 M

pH of solution = ?

Solution:

Formula:

pH = -log[H⁺]

by putting values,

pH = -log [ 0.003]

pH = 2.5

5 0
3 years ago
Which element is oxidized in the following reaction: 2FeCl₂ + Cl₂→2FeCl₃ if Fe goes from + 2 to +3 and Cl goes from 0 to −2?
Blizzard [7]

Answer:

Fe.

Explanation:

  • The element which is oxidized is the element that losses electrons and its oxidation state be more positive.
  • The element which is reduced is the element that gain electrons and its oxidation state be more negative.

<em> Fe goes from + 2 to +3, so, it is the element that is oxidized.</em>

<em></em>

3 0
3 years ago
The cation of the salt is sodium ion, and the anion is aurothiosulfate ion. Based on the chemical formula of the salt, what must
marta [7]

Answer:

3-

Explanation:

Sodium aurothiosulfate is a salt with the formula Na₃Au(S₂O₃)₂. The cation of the salt is sodium ion, and the anion is aurothiosulfate ion. We can determine the charge of the aurothiosulfate ion, considering that the sum of the positive and negative charges must be equal to the charge of the compound, which is zero.

3 × Na⁺ + 1 × Au(S₂O₃)₂ⁿ⁻ = 0

3 × +1 + 1 × Au(S₂O₃)₂ⁿ⁻ = 0

Au(S₂O₃)₂ⁿ⁻ = 3-

7 0
3 years ago
2. This graph shows the energy involved in a reaction involving two molecules. Describe what is happening or what the state of t
blagie [28]
If you think of it endothermic is when there is energy needed for the reaction to occur and exothermic is when the reaction releases energy
6 0
3 years ago
May you please check these please.
mihalych1998 [28]
I think you have the first three right
The last one should be increase, if there’s more reactants the particles are more likely to collide so the rate of reaction would increase, let me know if you want me to try explain that in an easier way aha
6 0
3 years ago
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