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matrenka [14]
2 years ago
10

Cultures glowing under UV light annotate transformation of the original bacteria with the pGLO plasmid. Bacteria without the pGL

O plasmid will neither glow under UV light nor be ampicillin resistant. If this is true, how can we have healthy, non-glowing, non-ampicillin resistant E. coli colonies growing on the LB/Amp/Ara plate
Chemistry
1 answer:
victus00 [196]2 years ago
7 0

Answer: Contamination

Explanation: This is a known contrainst when performing transformation in the lab. Normally the Pglo plasmid asides glowing under UV, possess a Amp gene that codes for resistance to the antibiotics Ampicillin. Transform cells will survive while untransformed cells will normally die. If untransformed (non glowing, non Ampicillin resistant) cells thrive in the medium, it is definitely a case of contamination. Start again but this time, disinfect appropriately bad be cautious of any potential contamination.

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Cobalt III hydroxide and nitric acid react according to the following balanced equation:
kati45 [8]
The mass of cobalt (III) needed is
m = 5.2 L (0.42 mol/L) ( 93 g/mol)
m = 97.65 g

The volume of nitric acid needed is
V = 5.2 L (0.42 mol/L) (3 mol / 1 mol) (1000 mL/1.6 mol)
V = 1968.75 mL

The moles of water produced is
n = 5.2 L (0.42 mol/L) (3 mol / 1 mol)
n = 3.15 moles
6 0
3 years ago
If 3.8 moles of zinc metal react with 6.5 moles of silver nitrate, how many moles of silver metal can be formed, and how many mo
bulgar [2K]

<u>Answer:</u> 6.5 moles of silver metal is formed in the given chemical reaction. The moles of excess reagent left are 0.55 moles.

<u>Explanation:</u>

To calculate the moles of silver formed and the moles of excess reagent left after the reaction, we need to balance the equation first and need to find the limiting and excess reagent.

The balanced chemical equation is:

Zn+2AgNO_3\rightarrow Zn(NO_3)_2+2Ag

By Stoichiometry:

2 moles of Silver nitrate reacts with 1 mole of Zinc metal

So, 6.5 moles of silver nitrate will react with = \frac{1}{2}\times 6.5=3.25moles of zinc metal

The required amount of zinc metal is less than the given amount of zinc metal,  hence, it is considered as an excess reagent.

Therefore, silver nitrate is the limiting reagent because it limits the formation of product.

By Stoichiometry of the reaction:

2 moles of silver nitrate produces 2 moles of silver metal

So, 6.5 moles of silver nitrate will produce = \frac{2}{2}\times 6.5=6.5moles of silver metal.

Number of moles of excess reagent left after the completion of reaction = (3.8 - 3.25)moles = 0.55 moles

Hence, 6.5 moles of silver metal is formed in the given chemical reaction. The moles of excess reagent left are 0.55 moles.

7 0
3 years ago
What is the difference between hypertonic and hypotonic
Bess [88]
Answer: A solution will be hypertonic to a cell if its solute concentration is higher than that inside the cell, and the solutes cannot cross the membrane. If a cell is placed in a hypotonic solution, there will be a net flow of water into the cell, and the cell will gain volume.

Picture:
Meaning of hypertonic: (1): having a higher osmotic pressure than a particular fluid, typically a body fluid or intracellular fluid. (2) of or in a state of abnormally high muscle tone.

5 0
2 years ago
Which reactant (X) is missing from the equation shown?<br> X+ PO4 → H3PO4
insens350 [35]

Answer:

H

Explanation:

b/c the polyatomic PO4¯³

have 3 valance number

3 0
2 years ago
How did music change during medieval, renaissance and baroque eras​
Vilka [71]

Answer:

usage of trills and vibrato

Explanation:

earlier music used trills while later styles would use vibrato.

7 0
3 years ago
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