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matrenka [14]
3 years ago
10

Cultures glowing under UV light annotate transformation of the original bacteria with the pGLO plasmid. Bacteria without the pGL

O plasmid will neither glow under UV light nor be ampicillin resistant. If this is true, how can we have healthy, non-glowing, non-ampicillin resistant E. coli colonies growing on the LB/Amp/Ara plate
Chemistry
1 answer:
victus00 [196]3 years ago
7 0

Answer: Contamination

Explanation: This is a known contrainst when performing transformation in the lab. Normally the Pglo plasmid asides glowing under UV, possess a Amp gene that codes for resistance to the antibiotics Ampicillin. Transform cells will survive while untransformed cells will normally die. If untransformed (non glowing, non Ampicillin resistant) cells thrive in the medium, it is definitely a case of contamination. Start again but this time, disinfect appropriately bad be cautious of any potential contamination.

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The concentration of protein in a urine sample is calculated to be 2.77 μg/mL. What is the concentration of this solution in uni
maxonik [38]

Answer:

The concentration of this solution in units of pounds per gallon is 2.776*10^{-5} \frac{lb}{gal}

Explanation:

Units of measurement are established models for measuring different quantities. The conversion of units is the transformation of a quantity, expressed in a certain unit of measure, into an equivalent one, which may or may not be of the same system of units.

In this case, the conversion of units is carried out knowing that 1 μg are equal to 2.205*10⁻⁹ Lb and  1 mL equals 0.00022 Gallons. So

2.77 \frac{ug}{mL} = \frac{2.77 ug}{mL}

If 1 μg equals 2.205*10⁻⁹  lb, 2.77 μg how many lb equals?

lb=\frac{2.77ug*2.205*10^{-9}lb }{1ug}

lb=6.10785*10⁻⁹

So, 2.77 μg= 6.10785*10⁻⁹ lb

Then:

2.77 \frac{ug}{mL} = \frac{2.77 ug}{mL}=\frac{6.10785*10^{-9}lb }{mL} =\frac{6.10785*10^{-9}lb }{0.00022 gal} =\frac{6.10785*10^{-9}lb }{0.00022 gal}

You get:

2.77 \frac{ug}{mL} = 2.776*10^{-5} \frac{lb}{gal}

<u><em>The concentration of this solution in units of pounds per gallon is </em></u>2.776*10^{-5} \frac{lb}{gal}<u><em></em></u>

6 0
3 years ago
The teacher tells your group to make a stock solution of sodium chloride, and then diluting it to
sergejj [24]

The idea here is that you need to figure out how many moles of magnesium chloride,

MgCl

2

, you need to have in the target solution, then use this value to determine what volume of the stock solution would contain this many moles.

As you know, molarity is defined as the number of moles of solute, which in your case is magnesium chloride, divided by liters of solution.

c

=

n

V

So, how many moles of magnesium chloride must be present in the target solution?

c

=

n

V

⇒

n

=

c

⋅

V

n

=

0.158 M

⋅

250.0

⋅

10

−

3

L

=

0.0395 moles MgCl

2

Now determine what volume of the target solution would contain this many moles of magnesium chloride

c

=

n

V

⇒

V

=

n

c

V

=

0.0395

moles

3.15

moles

L

=

0.01254 L

Rounded to three sig figs and expressed in mililiters, the volume will be

V

=

12.5 mL

So, to prepare your target solution, use a

12.5-mL

sample of the stock solution and add enough water to make the volume of the total solution equal to

250.0 mL

.

This is equivalent to diluting the

12.5-mL

sample of the stock solution by a dilution factor of

20

.

3 0
2 years ago
If 20 atoms of aluminum react with 45 molecules of chlorine gas, which reactant is limiting and how many more atoms/molecules wo
saul85 [17]
Al:ch2 an molar ratio.

2:3 and an x. X is 30.  20/2 = 10 so an answer is 

45/3 = 15. Both ratios are used 

45 - 30 = 15 CL2 left.

Your Welcome :)

4 0
3 years ago
Read 2 more answers
A saturated solution of calcium hydroxide contains 1.85 g of solute in 100. mL of solution. What is its molarity
Leona [35]

Answer:

M=0.250M

Explanation:

Hello!

In this case, since the molarity of a solution is computed by dividing the moles of solute by the volume of solution in liters, we first need to compute the moles of solute knowing that the molar mass of calcium hydroxide is 74.1 g/mol as follows:

n=1.85g*\frac{1mol}{74.1g}=0.0250mol

Next, since the 100-mL solution is also expressed in liters by 0.100 L, we directly compute the molarity as shown below:

M=\frac{0.0250mol}{0.100L}\\\\M=0.250M

Which is expressed in molar units that are mol/L.

Best regards!

8 0
3 years ago
5. Complete and balance an equation for each reaction:
Kipish [7]

CaI₂ + Hg(NO₃)₂ --------->HgI₂ + Ca(NO3)2

2Al + 3Cl₂ --------->2AlCl3

Ag + HCl ------->AgCl + H2

C2H2 + 5O2 --------> 4CO2 + 2H2O

MgCl₂ --------->Mg + Cl2

6 0
3 years ago
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