Agar is used to assist establish an anaerobic environment that promotes nitrate reduction.
Nitrate Reduction test:
- The nitrate in the broth is converted to nitrite by organisms that can produce the nitrate reductase enzyme, which can then be further converted to nitric oxide, nitrous oxide, or nitrogen.
- Anaerobic respiration and denitrification are two processes that can convert nitrate to a variety of compounds.
- While denitrification only reduces nitrate to molecular nitrogen, anaerobic respiration employs nitrate as the bacterium's final electron acceptor, reducing it to a range of chemicals.
- The nitrate reduction test is based on the detection of nitrite and its capacity to produce a red precipitate (prontosil), which is a water-soluble azo dye, when it combines with sulfanilic acid to create a complex (nitrite-sulfanilic acid).
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Mass= number of moles multiply Mass of one mole
= 24.3 multiplied by 4
= 96.2
Amm=(%of a × mass of a + %of b ×mass of b +%of c × mass of c)/100
At 37 degrees celsius, atoms of a He (g) sample would have the greatest average Kinetic energy.
