Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Standardized means that a specific amount EDTA is added to a specific volume of distilled water. Water hardness is determined by the the amount of a standard EDTA solution to change the color of the water from red to blue. For example if one added the correct amount of EDTA to twice the volume of distilled water the solution would be weak. Titration of the hard water would give a erroneous high result.
The possible number and location of all subatomic are one of them is electrically neutral, while the other has a stable electronic configuration.
<h3>What are subatomic particles?</h3>
Subatomic particles are those particles that are present inside the atoms. They are electron, neutron, and proton. They are charged particles, protons are positively charged, electrons are negatively charged and neutrons are neutral.
The protons and electrons totally contribute to the atomic mass of the elements.
Thus, the subatomic particles are electrically neutral and stable to electronic configurations.
To learn more about subatomic particles, refer to the below link:
brainly.com/question/13303285
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