The standard curve was made by spectrophotographic analysis of equilibrated iron(III) thiocyanate solutions of known concentrati
on. You are asked to analyze a Fe(SCN)2+ solution with an unknown concentration and an absorbance value of 0.410 . The slope-intercept form of the equation of the line is y=4541.6x+0.0461 . The unknown was analyzed on the same instrument as the standard curve solutions at the same temperature. What is the Fe3+ concentration of the unknown solution?
Molar concentration of the Fe³⁺ in the unknown solution is 8.01x10⁻⁵M.
Explanation:
When you make a calibration curve in a spectrophotographic analysis you are applying the Lambert-Beer law that states the concentration of a compound is directely proportional to its absorbance:
A = E*l*C
<em>Where A is absorbance, E is molar absorption coefficient, l is optical path length and C is molar concentration</em>
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Using the equation of the line you obtain:
y = 4541.6X + 0.0461
<em>Where Y is absorbance and X is concentration -We will assume concentration is given in molarity-</em>
As absorbance of the unknown is 0.410:
0.410 = 4541.6X + 0.0461
X = 8.01x10⁻⁵M
<h3>Molar concentration of the Fe³⁺ in the unknown solution is 8.01x10⁻⁵M.</h3>
