Hey there!:
From the given data ;
Reaction volume = 1 mL , enzyme content = 10 ug ( 5 ug in 2 mg/mL )
Enzyme mol Wt = 45,000 , therefore [E]t is 10 ug/mL , this need to be express as "M" So:
[E]t in molar = g/L * mol/g
[E]t = 0.01 g/L * 1 / 45,000
[E]t = 2.22*10⁻⁷
Vmax = 0.758 umole/min/ per mL
= 758 mmole/L/min
=758000 mole/L/min => 758000 M
Therefore :
Kcat = Vmax/ [E]t
Kcat = 758000 / 2.2*10⁻⁷ M
Kcat = 3.41441 *10¹² / min
Kcat = 3.41441*10¹² / 60 per sec
Kcat = 5.7*10¹⁰ s⁻¹
Hence kcat of xyzase is 5.7*10¹⁰ s⁻¹
Hope that helps!
Answer:
Nitrogen and Oxygen make up 99% of the Earths atmosphere. Then Argon makes up the rest of the atmosphere.
If he was the primary scientist doing it as he did alot of the heavy lifting then yes its ok, but i also think how the others should also me at least mentioned. Or they could just not name the experiment by a person just so its not too biased
Answer:
The intermediate is carbocation.
Explanation:
The reaction of alkene with an acid to give alcohol is an electrophilic addition reaction.
In electrophilic reaction, the alkene is being attacked by an alkene.
in case of aqueous acid reaction with alkene , the electrophile is [H⁺].
When the proton attacks the alkene it generates carbocation.
The carbocation generated is formed based on stability of carbocation.
The mechanism is shown in the figure.
