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leva [86]
3 years ago
9

Assuming that the experiments performed in the absence of inhibitors were conducted by adding 5 μl of a 2 mg/ml enzyme stock sol

ution to an assay mixture with a 1 ml total volume, and taking into account that xyzase is a monomeric enzyme with a molecular mass of 45,000 daltons, what is the kcat of xyzase given in s-1 (give the answer in two significant figures)? to see a sample kcat calculation, click on hint in the lower left corner to open the lower panel.
Chemistry
1 answer:
prohojiy [21]3 years ago
4 0

Hey there!:

From the given data ;

Reaction  volume = 1 mL , enzyme content = 10 ug ( 5 ug in 2 mg/mL )

Enzyme mol Wt = 45,000 , therefore [E]t is 10 ug/mL , this need to be express as "M" So:

[E]t in molar  = g/L * mol/g

[E]t  = 0.01 g/L * 1 / 45,000

[E]t = 2.22*10⁻⁷

Vmax = 0.758 umole/min/ per mL

= 758 mmole/L/min

=758000 mole/L/min => 758000 M

Therefore :

Kcat = Vmax/ [E]t

Kcat = 758000 / 2.2*10⁻⁷ M

Kcat = 3.41441 *10¹² / min

Kcat = 3.41441*10¹² / 60 per sec

Kcat = 5.7*10¹⁰ s⁻¹

Hence   kcat of   xyzase is  5.7*10¹⁰ s⁻¹


Hope that helps!



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A buffer solution is made that is 0.347 M in H2C2O4 and 0.347 M KHC2O4.
irga5000 [103]

Answer:

1. pH = 1.23.

2. H_2C_2O_4(aq) +OH^-(aq)\rightarrow HC_2O_4^-(aq)+H_2O(l)

Explanation:

Hello!

1. In this case, for the ionization of H2C2O4, we can write:

H_2C_2O_4\rightleftharpoons HC_2O_4^-+H^+

It means, that if it is forming a buffer solution with its conjugate base in the form of KHC2O4, we can compute the pH based on the Henderson-Hasselbach equation:

pH=pKa+log(\frac{[base]}{[acid]} )

Whereas the pKa is:

pKa=-log(Ka)=-log(5.90x10^{-2})=1.23

The concentration of the base is 0.347 M and the concentration of the acid is 0.347 M as well, as seen on the statement; thus, the pH is:

pH=1.23+log(\frac{0.347M}{0.347M} )\\\\pH=1.23+0\\\\pH=1.23

2. Now, since the addition of KOH directly consumes 0.070 moles of acid, we can compute the remaining moles as follows:

n_{acid}=0.347mol/L*1.00L=0.347mol\\\\n_{acid}^{remaining}=0.347mol-0.070mol=0.277mol

It means that the acid remains in excess yet more base is yielded due to the effect of the OH ions provided by the KOH; therefore, the undergone chemical reaction is:

H_2C_2O_4(aq) +OH^-(aq)\rightarrow HC_2O_4^-(aq)+H_2O(l)

Which is also shown in net ionic notation.

Best regards!

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