1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
leva [86]
3 years ago
9

Assuming that the experiments performed in the absence of inhibitors were conducted by adding 5 μl of a 2 mg/ml enzyme stock sol

ution to an assay mixture with a 1 ml total volume, and taking into account that xyzase is a monomeric enzyme with a molecular mass of 45,000 daltons, what is the kcat of xyzase given in s-1 (give the answer in two significant figures)? to see a sample kcat calculation, click on hint in the lower left corner to open the lower panel.
Chemistry
1 answer:
prohojiy [21]3 years ago
4 0

Hey there!:

From the given data ;

Reaction  volume = 1 mL , enzyme content = 10 ug ( 5 ug in 2 mg/mL )

Enzyme mol Wt = 45,000 , therefore [E]t is 10 ug/mL , this need to be express as "M" So:

[E]t in molar  = g/L * mol/g

[E]t  = 0.01 g/L * 1 / 45,000

[E]t = 2.22*10⁻⁷

Vmax = 0.758 umole/min/ per mL

= 758 mmole/L/min

=758000 mole/L/min => 758000 M

Therefore :

Kcat = Vmax/ [E]t

Kcat = 758000 / 2.2*10⁻⁷ M

Kcat = 3.41441 *10¹² / min

Kcat = 3.41441*10¹² / 60 per sec

Kcat = 5.7*10¹⁰ s⁻¹

Hence   kcat of   xyzase is  5.7*10¹⁰ s⁻¹


Hope that helps!



You might be interested in
What is the hydronium ion concentration of a 0.100 M acetic acid solution with Ka = 1.8 × 10-5? The equation for the dissociatio
evablogger [386]

Answer:

1.3×10⁻³ M

Explanation:

Hello,

In this case, given the dissociation reaction of acetic acid:

CH_3CO_2H(aq) + H_2O(l) \rightleftharpoons H_3O^+(aq) + CH_3CO_2^-(aq)

We can write the law of mass action for it:

Ka=\frac{[H_3O^+][CH_3CO_2^-]}{[CH_3CO_2H]}

Of course, excluding the water as heterogeneous substances are not included. Then, in terms of the change x due to the dissociation extent, we are able to rewrite it as shown below:

1.8x10^{-5}=\frac{x*x}{0.100-x}

Thus, via the quadratic equation or solve, we obtain the following solutions:

x_1=-0.00135M\\x_2=0.00133M

Obviously, the solution is 0.00133M which match with the hydronium concentration, thus, answer is: 1.3×10⁻³ M in scientific notation.

Regards.

8 0
3 years ago
Read 2 more answers
A balloon is filled with 35.0 L of helium in the morning when the temperature is 35.00 oC.
nexus9112 [7]

Answer: V = 33.9 L

Explanation: We will use Charles Law to solve for the new volume.

Charles Law is expressed in the following formula. Temperatures must be converted in Kelvin.

V1 / T1 = V2 / T2 then derive for V2

V2 = V1 T2 / T1

= 35 L ( 308 K ) / 318 K

= 33.9 L

5 0
3 years ago
How many molecules are in 2.50 moles of co2
zmey [24]

Answer:

1.51 \times 1024 \:  \: molecules

4 0
2 years ago
Read 2 more answers
How many levels will argon have?
Varvara68 [4.7K]
They have about 4 or 6
7 0
3 years ago
How much energy is needed to completely boil a 5.05g sample of water?
Keith_Richards [23]

Given what we know, we can confirm that the amount of heat energy that would be required in order to boil 5.05g of water is that of 11.4kJ of heat.

<h3>Why does it take this much energy to boil the water?</h3>

We arrive at this number by taking into account the energy needed to boil 1g of water to its vaporization point. This results in the use of 2260 J of heat energy. We then take this number and multiply it by the total grams of water being heated, in this case, 5.05g, which gives us our answer of 11.4 kJ of energy required.

Therefore, we can confirm that the amount of heat energy that would be required in order to boil 5.05g of water is that of 11.4kJ of heat.

To learn more about the behavior of water visit:

brainly.com/question/1416592?referrer=searchResults

8 0
2 years ago
Other questions:
  • Certain bacteria live in plant roots and receive sugars from the plants. The bacteria provide a source of useful nitrogen for th
    13·2 answers
  • WORTH 53 POINTS AND BRAINLIEST!!!
    6·2 answers
  • Ptrpared a solution of sodium chloride (NaCl) by measuring out 49.Umol of sodium chloride into 150. ML. Volumetric flask and fil
    13·1 answer
  • Referring to an activity series, which of the following combinations of reactants would not produce a successful single-replacem
    15·2 answers
  • ILL GIVE BRAINLIST Mrs. Kucalaba downloaded some science music from the Internet onto her iPhone. She listened to the science mu
    6·1 answer
  • The energy diagram illustrated in the model corresponds to what student B
    12·1 answer
  • This graph compares the amounts of three different substances that can dissolve in 100 g of water at temperatures between 0° C a
    14·2 answers
  • What do alkali metals do not include
    6·1 answer
  • Robert Boyle and Robert Hooke worked together and discovered some important physics concepts. Their work lead to the formation o
    9·2 answers
  • A sample of neon gas in a bulb is at 149.05 °C and 349.84 kPa. If the pressure drops
    15·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!