How many pairs of legs do insects have? five four three
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The Trex-Binding Factor in Muscle Creatine Kinase Enhancer has been identified and described below.
The Muscle Creatine Kinase Enhancer contains a positive control site called Transcriptional Regulatory Element X (Trex). The Trex site is critical for MCK expression in skeletal and cardiac muscle, according to research using transgenic animals and cell culture. Quantitative proteomics was used to identify the Trex-binding factor (TrexBF) as Six a homeodomain transcription factor of the Six/sine oculi's family, from a background of about
copurifying proteins, following selective enrichment for TrexBF using magnetic beads coupled to oligonucleotides containing either wild-type or mutant Trex sites. We showed that Six4 is TrexBF in mouse skeletal myocytes and embryonic day 10 chick skeletal and cardiac muscle, but Six
is the primary TrexBF in adult mouse heart, using gel shift assays and Six-specific antisera. Six
transactivates in co transfection investigations.
Learn more about Trex-Binding Factor here.
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