Question

MDCK and A549 cell cultures were grown in complete minimum essential medium (MEM) with 10% fetal bovine serum (FBS), 100 U/mL penicillin G, 100 ug/mL streptomycin sulfate, and 2mM glutamine. Your stock penicillin is 10,000 U/mL, your streptomycin is 10 mg/mL and glutamine is 200 mM. You need 25 mL of cell culture in a 250 mL canted neck flask. How much of each stock solution will you add? 1.6 x 10 ^6 U = 1 g Pen G.

Answer 1

Answer:

Used the C1V1 = C2V2 method for calculations

Explanation:

Component      Calculation       Volume of Stock to be added

FBS:             100*V1 = 10*25                  2.5 mL

Glutamine:     200*V1 = 2*25                  0.25 mL

Penicillin:      10000V1 = 100*25          0.25 mL

Streptomycin:     10000V1 = 100*25            0.25 mL

NB: C1 = Stock Concentration; V1 = Volume of stock required; C2 = Final concentration required; V2 = Final volume required