1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
ddd [48]
3 years ago
7

MDCK and A549 cell cultures were grown in complete minimum essential medium (MEM) with 10% fetal bovine serum (FBS), 100 U/mL pe

nicillin G, 100 ug/mL streptomycin sulfate, and 2mM glutamine. Your stock penicillin is 10,000 U/mL, your streptomycin is 10 mg/mL and glutamine is 200 mM. You need 25 mL of cell culture in a 250 mL canted neck flask. How much of each stock solution will you add? 1.6 x 10 ^6 U = 1 g Pen G.
Biology
1 answer:
UkoKoshka [18]3 years ago
3 0

Answer:

Used the C1V1 = C2V2 method for calculations

Explanation:

Component      Calculation       Volume of Stock to be added

FBS:             100*V1 = 10*25                  2.5 mL

Glutamine:     200*V1 = 2*25                  0.25 mL

Penicillin:      10000V1 = 100*25          0.25 mL

Streptomycin:     10000V1 = 100*25            0.25 mL

NB: C1 = Stock Concentration; V1 = Volume of stock required; C2 = Final concentration required; V2 = Final volume required

You might be interested in
The structure that is in physical contact with the auditory receptor stereocilia is the
ohaa [14]

Answer:

The structure that is in physical contact with the auditory receptor stereocilia is the tectorial membrane.

Explanation:

The tectorial membrane is a structure or a thin layer of tissue that is floating in the endolymph on the stereocilia of the ciliated cells. Ciliated cells, which are sensory cells attached to the surface of the basilar membrane.

When the ciliated cells move up and down, microscopic projections similar to sows (known as stereocilia), which are located above the ciliated cells, cause the cilia to lean towards the side wall of the cochlea. This inclination makes that some channels that look like pores open, which are at the tips of the stereocilia. When this happens, certain chemicals enter the cells, thus generating an electrical signal. The inner ciliated cell is activated, probably by a direct contact of its stereocilia with the Hensen band of the tectorial membrane, the synapse between the ciliated cell and the auditory nerve fibers, send a message to the brain, which converts it into sounds that we can recognize and understand.

8 0
3 years ago
Jumble letter can you help me plsss​
sveticcg [70]
<h2>Answer</h2>

  1. Wind
  2. Sunlight
  3. Plants
  4. Soil
  5. Coal
  6. Minerals
  7. Water
  8. Animals

\\

In short it's natural resources.

3 0
2 years ago
What role does the MutL protein play in DNA repair? (5 POINTS) It performs a proofreading function, similar to DNA polymerase, a
Zinaida [17]

Answer:

It recognizes and binds to a pair of "mismatched" nucleotides, preventing their translation.

Explanation:

Mut L protein is involved in mismatch DNA repair. MutL protein is complexed with MutS protein and the MutL-MutS complex recognizes all the mismatched base pairs present in the newly formed DNA strand. The complex can not recognize the "C-C" pairs. MutH protein joins the complex.

The MutH protein also has a site-specific endonuclease activity and cleaves the unmethylated DNA strand towards the 5' end of the guanine base in the GATC sequence to mark the strand for DNA repair. In this way, MutL protein, along with MutS and MutH proteins mark the mismatched DNA bases for repair so that they are not translated into a faulty protein.

5 0
3 years ago
what variable is measured in a experiment? A. independent variable B. experimental variable c. Dependent variable
Damm [24]
C. Dependent variable

The independent variable is what you want to change (ex amount of light given to plants)

The dependent variable is what changes because of the independent variable (ex the height of the plants)

So Independent is what you change, Dependent is what you measure
5 0
3 years ago
What 3 factors can have an effecct on enzymes? What is a lock and key model? Help plz
svlad2 [7]

concentration of the enzyme, concentration of the substrate, and temperature. However there are more than three.  A lock and key analogy is used when explaining the specific action of an enzyme with a single substrate.

5 0
3 years ago
Other questions:
  • When you rode a bicycle, when is friction helpful and when is it not helpful? Explain
    10·1 answer
  • The part of the Earth that is made up of plates is the
    11·1 answer
  • What value is closest to the mass of the atom? 4 amu 6 amu 10 amu 14 amu
    5·2 answers
  • Help pleeeeeeeeeeeeeeeaaaaaaasssssseeeeeee.
    14·1 answer
  • Which type of tissue functions as an absorber of digested nutrients?
    7·2 answers
  • Shelly and Jason are siblings, but Shelley looks like their father while Jason looks like their mother. Which of the following s
    5·2 answers
  • Alex wants to know if a fossil he found was closely related to cats. some likely features of the fossil that can help him reach
    5·1 answer
  • 1.
    8·1 answer
  • Natural selection, adaptations, genetic variation, and population evolution are all related in terms of changes in phenotypes ov
    15·1 answer
  • Two sisters are born from a father who has blue eyes and a mother who has brown eyes. One sister has blue eyes, and the other ha
    14·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!