Explanation:
B) After determining the optimum pH, they could vary the temperature of the environment to see if catalase is temperature specific
Enzymes are proteins which catalyze reactions by acting on substrates in order to speed up reactions- like the breakdown of large polysaccharides by amylase. Here, the enzyme catalase facilitates the breakdown of hydrogen peroxide into oxygen and hydrogen. Catalase specificity is affected by pH, temperature and the presence of inhibitors.
In temperatures beyond its optimal range, catalase may undergo changes to its physical structure called denaturation; when denatured, enzymes lose their ability to bind specifically to their substrate -i.e. substrate binding specificity is lost. H2O2 would no longer be able to bind to the active site, and thus would not be broken down.
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