When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
c. FADH2
Explanation:
FADH2 is an electron carrier that is generated in the reaction catalyzed by malic enzyme.
Answer:
Reasons why Maria will have tissue ischemia:
1. Because of evident problems with her blood circulation, this means insuficent blood flow. This shows in the edema she has.
2. The ischemia can lead to hypoxia because of the lack of oxygen in the blood stream to reach to the tissue cells.
Explanation:
Ischemia is a health problem characterized by the lack of oxygen that the bloods transports to the tissues in the body.
Hypoxia is related to the unsuficient oxigen supply gettin to a certain regions of the body or to the entire body.
Answer:
mRNA is transcribed from DNA.
The nucleotides in mRNA code for amino acids.
tRNA translates the mRNA sequence into an amino acid sequence so proteins can be made.
Explanation: FOR REAL THE FACTS
Explanation:
(3) active transport
The molecules would be moving against their concentration gradient from a region of low concentration to a region of high concentration.
While cells facilitate the transport of molecules via movement across the cell membrane, there many different mechanisms. These include passive diffusion, facilitated diffusion, and passive transport. However some very large molecules require specialized type of active transport, which requires energy in the form of ATP, in order to move substances across the membrane against their concentration gradient.
Active transport is a mediated process that requires an energy input and the use of specialized membrane proteins to move against the concentration gradient. These proteins require energy in the form of adenosine triphosphate or ATP in order to facilitate necessary conformational changes to the large protein molecules to alter the spatial location of the molecule. For instance, with Na+, K+ pumps in cell membranes.
Learn more about membrane components at brainly.com/question/1971706
Learn more about plasma membrane transport at brainly.com/question/11410881
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