Cell theory is the historic scientific theory, now universally accepted, that living organisms are made up of cells, that they are the basic structural/organizational unit of all organisms, and that all cells come from pre-existing cells.
(Hope this helps!!)
Gram's staining is a differential staining technique that employs a primary stain like crystal violet and a counter stain like safranin along with the decolourizing agent alcohol and a mordant called the Gram's iodine.
Iodine is a mordant added after the primary stain. It fixes the stain by combining with it to enchance the staining ability. This forms an insoluble crystal violet iodine complex appearing purple under the microscope. These microorganisms are classified as Gram positive.
If addition of iodine is skipped, crystal violet is not fixed on the slide and the insoluble complex is not formed. The cells are decolourized by alcohol and are stained by the counter stain safranin making the Gram positive cells wrongly indentified as Gram negative due to its pink colouration. Thus, the slide will show all the cells as pink coloured Gram negative cells.
Answer:
46 chromosomes
Explanation:
<em>I. G1 phase (Gap 1) - Cellular contents excluding the chromosomes, are duplicated. II. S phase (DNA Synthesis) - Each of the 46 chromosomes is duplicated by the cell.</em>
Answer:
In in vitro plant tissue culture, indolbutyric acid and other auxins are used to initiate root formation in a procedure called micropropagation. The micropropagation of plants is an asexual propagation or propagation technique that is based on the organogenetic potential of plant cells, which consists of cultivating in vitro on appropriate substrates, isolated cells, portions of yolk meristems, vegetative apices at the beginning of their development or microstaquillas. Small samples of plants used are called explants. Auxins such as indolbutyric acid can be used to cause mass formation of undifferentiated cells called corns. Callus formation is often used as a first step in the micropropagation process since, by exposure to certain auxin hormones, callus cells can be induced to form other tissues such as roots.
Indolbutyric acid is often used to promote the rooting of stakes. In a study in Camellia sinensis, the effect of three different auxins, indolbutyric acid, indolacetic acid and 1-naphthalenacetic acid on root formation was measured. According to the authors, indolbutyric acid produced a higher root yield compared to other auxins.9 This effect of indolbutyric acid is consistent with that found in other studies; This hormone is considered the most commonly used auxin for root formation, 10 because it is much more potent than indolacetic acid and other synthetic auxins.7
Jatropha curcas is a species in which there is an improvement in the quality of the rooting of the stakes with the addition of indolbutyric acid in the middle (longer root length, number of roots, percentage of rooted stakes, and dry root weight) , 11 although not always the effect on rooting is statistically significant.
