Answer and Explanation:
The steps for construction of the expression plasma
- STEP 1 : Introducing Notl and Hindlll confinement sites at the ends of the gene of interest by means of PCR
- STEP 2 :Digest the plasmid and DNA encoding gene with the Notl and Hindol confinement endonucleuses.
- STEP 3 : Ligate the cut plasmid and DNA fragment
- STEP 4 : Change the plasmid into E.coli for expression
- STEP 5 : Clean the protein by utilizing a column of immobilized
Answer: kinetochore
Spindle
fibers are aggregates of micro-tubules that move chromosomes during cell division. They attach to the kinetochore, a structure on chromatids, that when it's pulled separated sister chromatids. The kinetochore is a specialized protein complex found in the centromere of the chromosome.
According to the graph attached to support the task, I think that I recognized the correct answer, and I bet one negative consequence of using an insecticide is that it <span>selects for insecticide-resistant organisms. Actually, this option is the most impactful for environment, as the rest of the options pose as advantages. (besides the last one, but it does not make sense as the iformation doe not coincide with graph)
I hope you will agree with me.</span>
I think it is Centrioles
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