0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
<h3>What is dilution?</h3>
Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.
Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:
where:
- C1 = Initial concentration of enzyme
- C2 = Final concentration of enzyme
- V1 = Initial volume
- V2 = Final volume
For the enzyme dilution;
C1 = 1 mg/mL
C2 = 1/50 mg/ml = 0.02 mg/ml
V= ?
V2 = 5 ml
V1 = C2V2/C1
V1 = 0.02 * 5/1 = 0.1 mL
Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
Learn more about dilution at: brainly.com/question/24881505
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Physical change , the states are changing from liquid to gas
A. bacteria because its prokaryotic the others are eukaryotic
Answer:
The Third Law of thermodynamics states that the entropy of a pure substance in a perfect crystalline state at zero temperature is zero.
Answer:
Molality = 8.57 m
Explanation:
Given data:
Molarity of solution = 5.73 M
density = 0.9327 g/mL
Molality of solution = ?
Solution:
Molality = moles of solute / kg of solvent.
Kg of solvent:
Mass of 1 L solution = density× volume
Mass of 1 L solution = 0.9327 g/mL × 1000 mL
Mass of 1 L solution = 932.7 g
Mass of solute:
Mass of 1 L = number of moles × molar mass
Mass = 5.73 mol × 46.068 g/mol
Mass = 263.97 g
Mass of solvent:
Mass of solvent = mass of solution - mass of solute
Mass of solvent = 932.7 g - 263.97 g
Mass of solvent = 668.73 g
In Kg = 668.73 /1000 = 0.6687 Kg
Molality:
Molality = number of moles of solute / mass of solvent in Kg
Molality = 5.73 mol / 0.6687 Kg
Molality = 8.57 m
