Answer:
The International Date Line passes through the mid-Pacific Ocean and roughly follows a 180 degrees longitude north-south
line on the Earth. It is located halfway round the world from the prime meridian—the zero degrees longitude established in Greenwich
Multiply .800 moles of O2 by Avagadro's number divided by 1 mole. This will get rid of the moles on the bottom and leave you with molecules. So technically .800 times 6.02x10^23.
A foreign DNA molecule can be incorporated into a bacterial plasmid during a transformation reaction.
<h3>How to explain the reaction?</h3>
With the aid of two enzymes, ligase and restriction enzymes, a foreign DNA molecule can be incorporated into a bacterial plasmid during a transformation reaction. Each enzyme detects a target DNA sequence and cuts it nearby, while ligase aids in connecting the DNA. When two bits of DNA have complimentary bases, it facilitates their joining.
Plasmid and the insert fragment are both present in the microfuge tube, and they both have compatible sticky ends. However, the ligase has been denatured and is no longer active because the prior student left it outside rather than freezing it; despite this, we had already put the ligase into the tube. Ligase aids in binding the plasmid and insert fragments together, but because it is denatured in this instance, it will no longer be able to do so. As a result, no transformation process will take place. And since ligase links DNA fragments together by catalyzing the development of connections between the nearby nucleotides, the two fragments will not be able to unite.
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