Biuret reagent will indicate the presence of protein in a given sample. It is also known as the Piotrowski's test. This reagent consists of copper (II) sulfate and sodium hydroxide. It detects peptide bonds by the reaction of the copper ions in an alkaline solution. The copper ions would form violet colored complexes when peptide is present in the solution. From this test, concentration can be calculated since the intensity of the color depends on the amount of peptide bonds and according to the Beer-Lambert law concentration and the absorption of light is proportional. The concentration is calculated by a spectrophotometric technique at a wavelength of 540 nm.
Answer:
A pure solid is heated and turns into a pure liquid.
Explanation:
No colour change recorded, only change of state, hence this is a physical change - physical changes I.e. change of state and temperature are not chemical reactions.
Answer:
Explanation:
There are two heat transfers involved: the heat lost by the metal block and the heat gained by the water.
According to the Law of Conservation of Energy, energy can neither be destroyed nor created, so the sum of these terms must be zero.
Let the metal be Component 1 and the water be Component 2.
Data:
For the metal:
For the water:
