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spin [16.1K]
2 years ago
12

After conducting SDS-PAGE, you will conduct a staining/destaining procedure, which allows for visual identification of the prote

ins in your gel. This procedure requires the following staining solution: Coomassie Blue staining solution: 0.1% Coomassie Blue dye (dry powder) in 50% methanol, 10% acetic acid.
Required:
How to make 1 L of this solution?
Chemistry
1 answer:
expeople1 [14]2 years ago
8 0

Answer:

Add 700mL of water.

Explanation:

The technique of separation known as the Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis in which its acronym is SDS- PAGE is a very important aspect of Chemistry and Biochemistry as the technique make use of molar mass when it work in seperating proteins.

From the question we have the following parameters or information:

Case 1=> 50% of methanol which is the same as saying 50mL in 100mL of water.

Case 2=> 0.1% Comasse Blue dye (dry powder) which is the same as saying 0.1g in 100mL of water.

Case 3=> 10% acetic acid is the same as saying 10mL in 100mL of water.

Adding the three together, we have ;

50mL in 100mL of water + 0.1g in 100mL of water + 10mL in 100mL of water.

The volume of water in the three = 100 mL + 100mL + 100mL = 300mL.

Therefore, there is need to add 700mL of water to make 1L.

That's 700mL of water + 300mL = 1000mL = 1L

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Vladimir [108]

iron Bars form a solenoid when wrapped in a coil by a current carrying copper conducting wire .

copper coils generate a magnetic field when a current is passed through the coil . according to the helmholtz law.

6 0
2 years ago
3. Why do elements in group 1-17 react to form bonds?
iren [92.7K]

Answer:

Elements in the same group of the periodic table have the same number of valence electrons. These are the electrons in their outer energy level that can be involved in chemical reactions. ... All the elements in group 1 have just one valence electron. This makes them very reactive.

Explanation:

6 0
3 years ago
A brick has a mass of and the Earth has a mass of . Use this information to answer the questions below. Be sure your answers hav
Deffense [45]

Complete question

A brick has a mass of 4.0 kg and the Earth has a mass of 6.0 X 10²⁷ g. Use this information to answer the questions below. Be sure your answers have the correct number of significant digits. What is the mass of 1 mole of bricks? Round your answer to 2 significant digits. How many moles of bricks have a mass equal to the mass of the Earth? Round your answer to 2 significant digits.

Answer:

The mass of 1 mole of bricks = 24 X 10²⁶ g (2 significant digits), and

2.5 moles of bricks (2 significant digits) have a mass equal to the mass of the Earth.

Explanation:

Given mass of bricks = 4.0 kg

4.0kg of brick = 4000g

1 mole of any substance always contain  6.022 X 10²³ Avogadro's number

1 mole of bricks contains 6.022 X 10²³ bricks

Therefore, mass of 1 mole of brick = 6.022 X 10²³ X 4000g

= 4000 X 6.022 X 10²³ g

= 24088 X 10²³ g

The mass of 1 mole of bricks = 24 X 10²⁶ g (2 significant digits)

-----------------------------------------------------------------------------------------------------

⇒How many moles of bricks have a mass equal to the mass of the Earth?

Given mass of Earth = 6.0 X 10²⁷ g

24088 X 10²³ g --------> mass of 1 mole of bricks

6.0 X 10²⁷ g --------------> ?

= (6.0 X 10²⁷ g)/(24088 X 10²³ g)

= 2.4909 moles of bricks

≅ 2.5 moles of bricks ( 2 significant digits)

Therefore, 2.5 moles of bricks have a mass equal to the mass of the Earth

8 0
3 years ago
Which of the following is an example of a chemical change?
Vsevolod [243]

Answer:

D

Explanation:

It is D because once youve put together the ingredients and baked it you can not go in and taake out every ingredient and put it back to how it was before (Please also try to help me with my question it it about scaled copies, Please and thank you!)

6 0
2 years ago
Read 2 more answers
If i have 8 L of 0.25 M solution, how many miles of MgCl2 are present
qaws [65]

Answer:

2 moles

Explanation:

The following were obtained from the question:

Molarity = 0.25 M

Volume = 8L

Mole =?

Molarity is simply defined as the mole of solute per unit litre of solution. It is represented mathematically as:

Molarity = mole of solute/Volume of solution.

With the above equation, we can easily find the number of mole of MgCl2 present in 8 L of 0.25 M MgCl2 solution as follow:

Molarity = mole of solute/Volume of solution.

0.25 = mole of MgCl2 /8

Cross multiply to express in linear form

Mole of MgCl2 = 0.25 x 8

Mole of MgCl2 = 2 moles

Therefore, 2 moles of MgCl2 are present in 8 L of 0.25 M MgCl2 solution

4 0
3 years ago
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