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spin [16.1K]
2 years ago
12

After conducting SDS-PAGE, you will conduct a staining/destaining procedure, which allows for visual identification of the prote

ins in your gel. This procedure requires the following staining solution: Coomassie Blue staining solution: 0.1% Coomassie Blue dye (dry powder) in 50% methanol, 10% acetic acid.
Required:
How to make 1 L of this solution?
Chemistry
1 answer:
expeople1 [14]2 years ago
8 0

Answer:

Add 700mL of water.

Explanation:

The technique of separation known as the Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis in which its acronym is SDS- PAGE is a very important aspect of Chemistry and Biochemistry as the technique make use of molar mass when it work in seperating proteins.

From the question we have the following parameters or information:

Case 1=> 50% of methanol which is the same as saying 50mL in 100mL of water.

Case 2=> 0.1% Comasse Blue dye (dry powder) which is the same as saying 0.1g in 100mL of water.

Case 3=> 10% acetic acid is the same as saying 10mL in 100mL of water.

Adding the three together, we have ;

50mL in 100mL of water + 0.1g in 100mL of water + 10mL in 100mL of water.

The volume of water in the three = 100 mL + 100mL + 100mL = 300mL.

Therefore, there is need to add 700mL of water to make 1L.

That's 700mL of water + 300mL = 1000mL = 1L

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Starting with the unbalanced equation for the combustion of ethanol, C2H5OH(l)+O2(g)→CO2(g)+?H2O(l) what coefficient should be p
lianna [129]

Answer:

C2H5OH(l) + O2(g) → CO2(g) + ?H2O(l)

The coefficient should be 3

Explanation:

C2H5OH(l) + O2(g) → CO2(g) + ?H2O(l)

The chemical equation is a reaction between ethanol and oxygen . The reaction yield is carbon dioxide and water. Balancing the chemical equation requires one to make the number of atoms on the reactant side be equal to the number of atoms on the product side.

C2H5OH(l) + 3O2(g) → 2CO2(g) + 3H2O(l)

The hydrogen atom on the left hand side of the equation is 6. Therefore, the number of atom of hydrogen should be 6 on the right hand side. The number of oxygen atom on the left hand side is 7. Therefore, the number of Oxygen atom on the right hand side should  7 . Oxygen atom in carbon dioxide(right hand side) is 4 atoms, therefore, it requires 3 atoms of oxygen to make the  oxygen atoms 7.

Using 3 as the coefficient of water in the equation will make hydrogen 6 and oxygen 3 .Adding the 3 oxygen to the already 4 atoms of oxygen in carbon dioxide will make oxygen 7 atoms

 

6 0
2 years ago
In a water molecule, the shared electrons spend more time around
Gekata [30.6K]
Probably very negative or sightless negative
6 0
2 years ago
For all the chemistry people out there. Explain why Benzene is more stable that 1,3,5-hexatriene? ...?
Triss [41]
Benzene is more stable because it contains a ring and therefore an aromatic compound. On the other hand, 1,3,5-hexatriene, as a straight-chain alkene, is aliphatic. Aromatic compounds in general are more stable than their aliphatic counterparts because pi electrons are part of a conjugated system, meaning they are "shared" amongst all double-bonded atoms.










4 0
3 years ago
A 1.25 g sample of aluminum is reacted with 3.28 g of copper (II) sulfate. What is the limiting reactant?
KengaRu [80]

Answer:

d. Copper (II) sulfate

Explanation:

Given data:

Mass of Al = 1.25 g

Mass of CuSO₄ = 3.28 g

What is limiting reactant = ?

Solution:

Chemical equation:

2Al + 3CuSO₄   →   Al₂ (SO₄)₃ + 3Cu

Number of moles of Al:

Number of moles = mass/molar mass

Number of moles = 1.25 g/ 27 g/mol

Number of moles = 0.05 mol

Number of moles of CuSO₄:

Number of moles = mass/molar mass

Number of moles = 3.28 g/ 159.6 g/mol

Number of moles = 0.02 mol

now we will compare the moles of reactant with product.

               Al           :           Al₂ (SO₄)₃

                 2          :             1

               0.05       :          1/2×0.05=0.025 mol

                Al           :            Cu

                 2            :              3

               0.05         :            3/2×0.05 = 0.075 mol

         CuSO₄           :           Al₂ (SO₄)₃

                3             :             1

               0.02         :          1/3×0.02=0.007 mol

         CuSO₄           :            Cu

               3               :              3

               0.02         :              0.02

Less number of moles of reactants are produced by CuSO₄ thus it will act as limiting reactant.

4 0
3 years ago
How many moles are in 5.03 x 1023 molecules?
AlladinOne [14]

Answer:

Some formulas for calculating mole are

Mole = Mass/ Molar mass

Mole = no of particles / avogadros constant

NB : no of particles can be no of atoms , no of ions , or no of molecules 2. Avogadros number or constant = 6.02 times 10 ^23

so we will be using the second formula

Mole = no of particles / avogadros constant

Mole =  5.03 x 10 ^23/6.02 x10^23

Mole = 8.355x10^45

hope it helps :)

Explanation:

4 0
3 years ago
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