1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
spin [16.1K]
3 years ago
12

After conducting SDS-PAGE, you will conduct a staining/destaining procedure, which allows for visual identification of the prote

ins in your gel. This procedure requires the following staining solution: Coomassie Blue staining solution: 0.1% Coomassie Blue dye (dry powder) in 50% methanol, 10% acetic acid.
Required:
How to make 1 L of this solution?
Chemistry
1 answer:
expeople1 [14]3 years ago
8 0

Answer:

Add 700mL of water.

Explanation:

The technique of separation known as the Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis in which its acronym is SDS- PAGE is a very important aspect of Chemistry and Biochemistry as the technique make use of molar mass when it work in seperating proteins.

From the question we have the following parameters or information:

Case 1=> 50% of methanol which is the same as saying 50mL in 100mL of water.

Case 2=> 0.1% Comasse Blue dye (dry powder) which is the same as saying 0.1g in 100mL of water.

Case 3=> 10% acetic acid is the same as saying 10mL in 100mL of water.

Adding the three together, we have ;

50mL in 100mL of water + 0.1g in 100mL of water + 10mL in 100mL of water.

The volume of water in the three = 100 mL + 100mL + 100mL = 300mL.

Therefore, there is need to add 700mL of water to make 1L.

That's 700mL of water + 300mL = 1000mL = 1L

You might be interested in
What would be the freezing point of a 1.7 mole aqueous ethylene glycol solution? The freezing point depression constant for wate
nata0808 [166]

Answer:

-3.2^oC

Explanation:

In order to answer this question, we need to be familiar with the law of freezing point depression. The law generally states that mixing our solvent with some particular solute would decrease the freezing point of the solvent.

This may be expressed by the following relationship:

\Delta T_f=iK_fb

Here:

\Delta T_f=T_{initial}-T_{final} is the change in the freezing point of the solvent given its initial and final freezing point temperature values;

i is the van 't Hoff factor (i = 1 for non-electrolyte solutes and i depends on the number of moles of ions released per mole of ionic salt);

K_f is the freezing point depression constant for the solvent;

b=\frac{n_{solute}}{m_{solvent}} is molality of the solute, defined as a ratio between the moles of solute and the mass of solvent (in kilograms).

We're assuming that you meant 1.7-molal solution, then:

b=1.7 m

Given ethylene glycol, an organic non-electrolyte solute:

i=1

The freezing point depression constant:

K_f=1.86^oC/m

Initial freezing point of pure water:

T_{initial}=0.00^oC

Rearrange the equation for the final freezing point and substitute the variables:

T_f=T_o-iK_fb=0.00^oC-1\cdot1.86^oC/m\cdot1.7 m=-3.16^oC

8 0
3 years ago
Which of the following transition is considered the phase<br> change from water to steam?
Alexandra [31]

Answer:

the transition from water to steam is call evaporation.

Explanation:

water evaporates or vaporizes and turns into steam this typically happens when heat meets with the water. an example would be if you boil water steam will release from the pot, or when it rains, snows, etc (precipitation) the water evaporates and the ground dries.

4 0
3 years ago
Which taxonomic classification level is the largest or most broad?(1 point)
patriot [66]

According to the research, domain is the taxonomic classification level that is the largest or most broad.

<h3>What is domain?</h3>

It is the broadest taxonomic category in which known living things are classified.

In other words, it is the broadest category in which the different kingdoms of life can be organized, according to the classification models that are most widely accepted in the scientific community.

Therefore, we can conclude that according to the research, domain is the taxonomic classification level that is the largest or most broad.

Learn more about taxonomic classification here: brainly.com/question/13049455

#SPJ1

7 0
3 years ago
Which of the following elements has the lowest normal boiling point? 4 points A. Ne B. He C. Ar D. Kr
yanalaym [24]

Answer:

B. He

Explanation:

8 0
3 years ago
A partir de lo propuesto por Bohr en 1913, que postulado es correcto:
TEA [102]

Answer:

Primer postulado:

Así Bohr asumió que el átomo de hidrógeno puede existir solo en ciertos estados discretos, los cuales son denominados estados estacionarios del átomo. En el átomo no hay emisión de radiación electromagnética mientras el electrón no cambia de órbita.

Explanation:

4 0
3 years ago
Other questions:
  • I need the variables in the chart to be answered.
    7·1 answer
  • How many moles of potassium hydroxide are needed to completely react with 2.94 moles of aluminum sulfate according to the follow
    11·1 answer
  • SOMEONE PLEASE HELP ME!!!!!!!!!!!!!!!!!!!!
    5·1 answer
  • Isotopes of the same element differ in
    13·1 answer
  • What is the definition of work?
    12·2 answers
  • How many grams of Neon are there in a 346 mL container at 40.5°C and 842 torr?<br><br> With steps!
    8·1 answer
  • Which statement describes how heat is transferred by convection?
    15·1 answer
  • For the reaction below, which change would cause the equilibrium to shift to the right? EXPLAIN.
    12·1 answer
  • Which of the following corresponds to an alpha particle?
    14·1 answer
  • Which of these is a property that does NOT change for all objects of one substance?
    6·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!