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spin [16.1K]
3 years ago
12

After conducting SDS-PAGE, you will conduct a staining/destaining procedure, which allows for visual identification of the prote

ins in your gel. This procedure requires the following staining solution: Coomassie Blue staining solution: 0.1% Coomassie Blue dye (dry powder) in 50% methanol, 10% acetic acid.
Required:
How to make 1 L of this solution?
Chemistry
1 answer:
expeople1 [14]3 years ago
8 0

Answer:

Add 700mL of water.

Explanation:

The technique of separation known as the Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis in which its acronym is SDS- PAGE is a very important aspect of Chemistry and Biochemistry as the technique make use of molar mass when it work in seperating proteins.

From the question we have the following parameters or information:

Case 1=> 50% of methanol which is the same as saying 50mL in 100mL of water.

Case 2=> 0.1% Comasse Blue dye (dry powder) which is the same as saying 0.1g in 100mL of water.

Case 3=> 10% acetic acid is the same as saying 10mL in 100mL of water.

Adding the three together, we have ;

50mL in 100mL of water + 0.1g in 100mL of water + 10mL in 100mL of water.

The volume of water in the three = 100 mL + 100mL + 100mL = 300mL.

Therefore, there is need to add 700mL of water to make 1L.

That's 700mL of water + 300mL = 1000mL = 1L

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KHP is a monoprotic acid which provides one H+ ion. How would your results be affected if a diprotic acid (such as sulfuric acid
kari74 [83]

Explanation:

A.

In a diprotic acid, 2 moles of H+ ions is released. Therefore, number of moles of H+ in a diprotic acid = 2 × number of moles of H+ of monoprotic acid.

B.

Equation of the reaction

2NaOH + H2SO4 --> Na2SO4 + 2H2O

Number of moles of H2SO4 = molar concentration × volume

= 0.75 × 0.0105

= 0.007875 moles.

By stoichiometry, since 1 mole of H2SO4 reacts with 2 moles of NaOH. Therefore, number of moles of NaOH = 2 × 0.007875

= 0.01575 moles.

Molar concentration of NaOH = number of moles ÷ volume

= 0.01575 ÷ 0.0175

= 0.9 M of NaOH.

7 0
3 years ago
You drop an unknown substance that weighs 8.3g into a graduated cylinder with 6ml of water. The water rises to 8ml when you drop
valkas [14]

Answer:

<h3>The answer is 4.15 g/mL</h3>

Explanation:

The density of a substance can be found by using the formula

density =  \frac{mass}{volume} \\

From the question

mass of object = 8.3 g

volume = final volume of water - initial volume of water

volume = 8 - 6 = 2 mL

So we have

density =  \frac{8.3}{2}  \\

We have the final answer as

<h3>4.15 g/mL</h3>

Hope this helps you

7 0
3 years ago
How are carrot, an amoeba, and a bacterium alike
sineoko [7]
<span>They should all be eukaryotic.</span>
4 0
3 years ago
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Which of the following diagrams correctly shows the reaction pathway of a reaction that releases energy ?
zavuch27 [327]
The answer is diagram A.

Hope this helped. Good luck!
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Predict the outcome if a solution of yellow
Shkiper50 [21]

Answer: The solution will remain yellow.

Explanation:

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