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spin [16.1K]
3 years ago
12

After conducting SDS-PAGE, you will conduct a staining/destaining procedure, which allows for visual identification of the prote

ins in your gel. This procedure requires the following staining solution: Coomassie Blue staining solution: 0.1% Coomassie Blue dye (dry powder) in 50% methanol, 10% acetic acid.
Required:
How to make 1 L of this solution?
Chemistry
1 answer:
expeople1 [14]3 years ago
8 0

Answer:

Add 700mL of water.

Explanation:

The technique of separation known as the Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis in which its acronym is SDS- PAGE is a very important aspect of Chemistry and Biochemistry as the technique make use of molar mass when it work in seperating proteins.

From the question we have the following parameters or information:

Case 1=> 50% of methanol which is the same as saying 50mL in 100mL of water.

Case 2=> 0.1% Comasse Blue dye (dry powder) which is the same as saying 0.1g in 100mL of water.

Case 3=> 10% acetic acid is the same as saying 10mL in 100mL of water.

Adding the three together, we have ;

50mL in 100mL of water + 0.1g in 100mL of water + 10mL in 100mL of water.

The volume of water in the three = 100 mL + 100mL + 100mL = 300mL.

Therefore, there is need to add 700mL of water to make 1L.

That's 700mL of water + 300mL = 1000mL = 1L

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Describe a way to separate iron filings from sand.
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Use a magnet to separate the iron from the sand.
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How many molecules are in 69 grams of Na?
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Molar mass Na = 23.0 g/mol

1 mol ---- 23.0 g
n mol ---- 69 g

n = 69 / 23.0

n = 3.0 moles

1 mole -------- 6.02x10²³ molecules
3.0 moles ---- ?

3.0 *  6.02x10²³ / 1

= 1.806x10²⁴ molecules

hope this helps!




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2 years ago
Ethane is an alkane having two carbon atoms bound to hydrogen atoms. Recall what you have learned about the tetravalent nature o
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3 years ago
Write ionic equations for any three of the following:
worty [1.4K]

1.

The ionic equation is:

H⁺(aq) + NO₃⁻(aq) + Na⁺(aq) + OH⁻(aq) → Na⁺(aq) + NO₃⁻(aq) + H₂O(l)

HNO₃(aq) + NaOH(aq) → NaNO₃(aq) + H2O(l)

The required dissociations are

HNO₃(aq) → H⁺(aq) + NO₃⁻(aq)

NaOH(aq) → Na⁺(aq) + OH⁻(aq)

NaNO₃(aq) → Na⁺(aq) + NO₃⁻(aq)

So, the ionic equation is

H⁺(aq) + NO₃⁻(aq) + Na⁺(aq) + OH⁻(aq) → Na⁺(aq) + NO₃⁻(aq) + H₂O(l)

2.

The ionic equation is

H⁺(aq) + Cl⁻(aq) + K⁺(aq) + OH⁻(aq) → K⁺(aq) + Cl⁻(aq) + H₂O(l)

HCl(aq) + KOH(aq) → KCl(aq) + H2O(l)

The required dissociations are

HCl(aq) → H⁺(aq) + Cl⁻(aq)

KOH(aq) → K⁺(aq) + OH⁻(aq)

KCl(aq) → K⁺(aq) + Cl⁻(aq)

So, the ionic equation is

H⁺(aq) + Cl⁻(aq) + K⁺(aq) + OH⁻(aq) → K⁺(aq) + Cl⁻(aq) + H₂O(l)

3.

The ionic equation is

2H⁺(aq) + SO₄²⁻(aq) + Mg²⁺(aq) + 2OH⁻(aq) → Mg²⁺(aq) + SO₄²⁻(aq)

+ 2H₂O(l)

H₂SO₄(aq) + Mg(OH)₂(aq) → MgSO₄(aq) + 2H2O(l)

The required dissociations are

H₂SO₄(aq) → 2H⁺(aq) + SO₄²⁻(aq)

Mg(OH)₂(aq) → Mg²⁺(aq) + 2OH⁻(aq)

MgSO₄(aq) → Mg²⁺(aq) + SO₄²⁻(aq)

So, the ionic equation is

2H⁺(aq) + SO₄²⁻(aq) + Mg²⁺(aq) + 2OH⁻(aq) → Mg²⁺(aq) + SO₄²⁻(aq)

+ 2H₂O(l)

Learn more about ionic equations here:

brainly.com/question/11628165

5 0
2 years ago
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