The National ... reduces nausea and vomiting, although it is unclear how it works. This sentence provides specific evidence from a scientific organization.
Answer:
A conversion error between two measurement systems
Explanation:
Answer:
In a fruit fly experiment, grey body, normal winged (homozygous dominant) fruit flies were mated with black body, short winged (homozygous recessive) fruit flies. The F1 dihybrid females were then used in a test cross. If the genes are always linked and no crossing over occurs, what would be the predicted ratio in the F2 generation?
GG x bb = Gb, Gb, Gb and Gb F1 generation
grey body heterozygous offspring 4:0
Gb x Gb= GG, Gb, Gb, and bb F2 generation
3:1 three grey body fly and one black body fly
Explanation:
Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
Rotator cuff
The rotator cuff is a group of muscles that perform a variety of tasks such as flexion, abduction, internal rotation and external rotation. The muscles in this group are essential to all movement of the shoulder, making them very essential for upper body mobility. Namely, the muscles of the rotator cuff are supraspinatus, infraspinatus, teres minor and subscapularis.
