Question

Many organisms possessing many different lipases produce positive results on tributyrin agar. Is the inability of this medium to distinguish between these different enzymes a weakness in its specificity or its sensitivity? Explain

Answer 1

Explanation:

No, it's not, tributyrin agar's functions is to detect lipolytic microorganisms such as Staphylococci, Clostridia, Pseudomonas, etc. through the formation of clear zones surrounding the lipolytic colonies. It is classified as a differential medium because even though it can differentiate between lipases it can differentiate between an organism with the presence of lipases or not.

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Answer 2

Answer: See explanation below

Explanation:

Tributyrin agar Is the inability of this medium to distinguish between these different enzymes a weakness in its specificity or its sensitivity.

Lipases break down lipids (fats).

Tributyrin oil is a type of lipid called a triglyceride. Other lipase tests use different fat sources such as corn oil, olive oil, peanut oil, egg yolk, and soybean oil.

Lipase allows the organisms that produce it to break down lipids into smaller fragments. Triglycerides are composed of glycerol and three fatty acids. These get broken apart and may be converted into a variety of end-products that could be used by the cell in energy production or other process.

Tributyrin oil forms an opaque suspension in the agar. When an organism produces lipase and breaks down the tributyrin, a clear halo surrounds the areas where the lipase-producing organism has grown.