Injury in frontal lobe can cause to muscle weakness and language problems.
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
the shaft and the bone marrow
Explanation:
A hard outer layer called cortical (compact) bone, which is strong, dense and tough. 2. A spongy inner layer called trabecular (cancellous) bone. This network of trabeculae is lighter and less dense than compact bone.
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Answer:
the twenty second amendments to the united states
Explanation:
limits the number of times of a person is eligible for election to the office of president of the united states to two,and sets additional eligibility conditions for president who succeed to the unexpired terms of their predecessors
