Restriction enzymes and or restriction endonucleases are involved at recognizing specific sequence of nucleotides and cutting or splicing them at appropriate regions to produce fragments that can either be sticky ends or blunt ends depending on where they cut and the nature of nucleotides involved within the fragments. They play an important role in genetic engineering, as geneticists can use them for placing into extra chromosomal information and or content of plasmids in certain bacteria, from other sources, for instance antibiotics, grow and or produce many individual colonies of bacteria, isolate them and one would have many sequences for instance that can code for an antibiotic that can be extracted and used further. Assuming the bacteria's plasmid can take in that sequence.
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For example, delays in mitosis are often ascribed to 'activation' of the mitotic checkpoint, a descriptor that fails to recognize that the checkpoint by definition is active as the cell starts mitosis. Conversely, the completion of mitosis in the presence of misaligned chromosomes is often automatically interpreted to indicate a defective checkpoint, even though in the absence of critical testing alternative interpretations are equally likely. In this article, we define the critical characteristics of checkpoints and illustrate how confusion generated by the inconsistent use of terminology may impede progress by fostering claims that mean very different things to different researchers. We will illustrate our points with examples from the checkpoint that controls progression through mitosis
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