The use of mass spectrometry in protein identification has revolutionized the field of proteomics. Coupled to various affinity purification techniques, mass spectrometry is used to identify protein-protein interactions. This chapter looks at the use of these affinity purification techniques in the identification of protein interactions. If want to know more about silac mass spectrometry, more info can be reached at <em>https://www.creative-proteomics.com/services/silac-based-proteomics-analysis.htm</em>