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Darya [45]
3 years ago
13

(2pts) During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer A150 is 30mM

Tris (pH 8.8) and 150mM NaCl. Given 1L of 1.5M Tris and 500ml of 5M NaCl, how much of each stock do you need to make 250ml of the A150 buffer
Chemistry
1 answer:
torisob [31]3 years ago
6 0

Answer:

The answer is "20 \ mL"

Explanation:

Given:

Molarity= number of moles

because it is 1 Liter

\to \frac{0.03\ moles}{1.5 moles}=0.02\ L= 20 \ mL \ of\  Tris\\\\

therefore,

it takes 20 mL of Tris.

\to \frac{0.150 \ moles}{5\ moles} =0.03\ L\\\\

                     = 30 \ mL \ of\ Nacl

So, take 20 \ mL\ of\ NaCl.

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Explanation:

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                    Ka = 4.9 × 10^-10

               KaKb = Kw

4.9 × 10^-10 Kb = 1.00 × 10^-14

                   Kb = (1.00 × 10^-14)/(4.9 × 10^-10) = 2.05 × 10^-5

Now, we can set up an ICE table

                     CN^- + H2O ⇌ HCN + OH^-

I/(mol/L)      0.255                     0         0

C/(mol/L)       -x                        +x        +x

E/(mol/L)  0.255 - x                   x         x

Ka = x^2/(0.255 - x) = 2.05 × 10^-5

Check for negligibility

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        x = sqrt(5.20 × 10^-6)    = 2.28 × 10^-3

[OH^-] = x mol/L                     = 2.28 × 10^-3 mol/L

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