Question

(2pts) During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer A150 is 30mM Tris (pH 8.8) and 150mM NaCl. Given 1L of 1.5M Tris and 500ml of 5M NaCl, how much of each stock do you need to make 250ml of the A150 buffer

Answer 1

Answer:

The answer is "$20 \ mL$"

Explanation:

Given:

Molarity= number of moles

because it is 1 Liter

$\to \frac{0.03\ moles}{1.5 moles}=0.02\ L= 20 \ mL \ of\ Tris$

therefore,

it takes 20 mL of Tris.

$\to \frac{0.150 \ moles}{5\ moles} =0.03\ L$

                     $= 30 \ mL \ of\ Nacl$

So, take $20 \ mL\ of\ NaCl.$