1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Greeley [361]
2 years ago
14

How would you make 5mls of a solution that is 2.0% lactose and 0.1M SPG buffer from separate stock solutions that are 6% lactose

and 1M SPG in concentration
Chemistry
1 answer:
Aliun [14]2 years ago
7 0

Answer:

Check the explanation section.

Explanation:

The following steps should be followed orderly.

STEP ONE:

Use the dilution equation in the calculation of the volume for the stock solution. That is, C1 × V1 = C2 × V2.

Where C1 and C2 are the concentration of the stock solution and the diluted solution.

STEP TWO:

Put 6% of lactose and make sure to dilute it in order to make 2.0% lactose

and put it in Beaker A. Also, make sure to dilute the 1M to 0.1M SPG buffer in Beaker B.

STEP TWO:

Now, from beaker A containing 2% lactose, measure and remove 5.0 mL from it. Also, measure and remove 5.0 mL from beaker B containing 0.1M SPG.

So, in STEP TWO above we won't know how much water we need to use for dilution, thus, there is the need to make use of STEP ONE.

Therefore, from STEP ONE ABOVE, we have the dilution equation given as;

C1 × V1 = C2 × V2.

Hence, 6 × V1 = 2 × 5. Therefore, the volume needed from the stock solution, V1 = (2 × 5)/ 6 = 1.6 mL.

STEP THREE:

Now measure out 1.6 mL from the stock solution, that is 6% lactose and add it to 5mL of the diluted solution of 2% in beaker A into another container, say beaker C and add H2O to form SOLUTION X.

STEP FOUR:

Using the dilution equation again, Determine the the volume that is needed from 1M SPG.

C1 × V1 = C2 × V2.

V1 = ( 0.1 × 5)/ 1 = 0.5mL.

STEP FIVE:

measure 0.5mL out from the 1M SPG and 5 mL out of 0.1M SPG buffer and add water to it to form SOLUTION Y.

STEP SIX:

Now, mix solution X and solution Y together and take the required 5ml

You might be interested in
What is the energy of light with a wavelength of 468 nm? (The speed of light
marshall27 [118]

The answer for the problem is explained below.

The option for the answer is "D".

<u><em>Therefore the energy of the light is  4.25 × 10^-19 J</em></u>

Explanation:

Given:

wavelength (λ) = 468 nm = 468×10^-9 m

speed of light (c) = 3.00 x 10^8m/s

Planck's constant is 6.626 x 10^-34J·s

To solve:

energy of light (E)

We know,

E =(h×c) ÷ λ

E = ( 6.626 x 10^-34 ×  3.00 x 10^8) ÷ 468×10^-9

E = 4.25 × 10^-19 J

<u><em>Therefore the energy of the light is  4.25 × 10^-19 J</em></u>

6 0
3 years ago
How to calculate average mass in grams?
Dafna1 [17]

Explanation:

hope these help you answer

7 0
3 years ago
Convert 732.0 mmHg to atm
enot [183]

Answer:

The answer is

<h2>0.95 atm</h2>

Explanation:

To solve the question we use the following conversion

That's

1 mmHg \cong 0.0013 atm

So we have

If 1 mmHg \cong 0.0013 atm

Then 732 mmHg will be

732 × 0.0013 atm

We have the final answer as

<h3>0.95 atm</h3>

Hope this helps you

5 0
3 years ago
1. All ___matter______ is made of atoms that cannot be ______________________ or ____________________. 2. All _______________ of
marin [14]

Answer:

All the matter that is made of atoms that cannot be broken or beaten into thin sheet .2All matters of a given book are in mass and mixture

4 0
2 years ago
Show All Work For Brainliest
sattari [20]

Answer:

1) <u>16.8 L CO2</u>

<u>2) 36.96 L NH3</u>

<u>3) </u> <u>9.88 L CO2 </u>

<u>4) 56.99 L H2O</u>

Explanation:

How many liters of carbon dioxide gas will be produced when 75.0 g of calcium carbonate decomposes to form calcium oxide when at STP?

CaCO3 → CaO + CO2

Moles calcium carbonate = 75.0 grams / 100.09 g/mol

Moles calcium carbonate = 0.750 moles

For 1 mol CaCO3 we'll have 1 mol CaO and 1 mol CO2

For 0.750 moles CaCO3 we'll have 0.750 moles CO2

1 mol = 22.4 L

0.750 moles CO2 = 0.750 *22.4 L =<u> 16.8 L CO2</u>

2. Hydrogen gas reacts with 23.1 g of nitrogen gas to produce ammonia (NH3). What volume of ammonia will be produced at STP?

3H2 + N2 → 2NH3

Moles N2 = 23.1 grams / 28.0 g/mol

Moles N2 = 0.825 moles

For 3 moles H2 we need 1 mol N2 to produce 2 moles NH3

For 0.825 moles N2 we'll have 2*0.825 = 1.65 moles NH3

1 mol = 22.4 L

1.65 mol = 1.65 * 22.4 L = <u>36.96 L NH3</u>

<u />

<u />

3. Iron (III) oxide reacts with carbon monoxide to form iron and carbon dioxide. How many liters of carbon dioxide will be produced from 23.5 g of iron (III) oxide when at STP?

Fe2O3 + 3CO → 2Fe + 3CO2

Moles Fe2O3 = 23.5 grams / 159.69 g/mol

Moles Fe2O3 = 0.147 moles

For 1 mol Fe2O3 we need 3 moles CO to produce 2 moles Fe and 3 moles CO2

For 0.147 moles Fe2O3 we'll have 3*0.147 = 0.441 moles CO2

1 mol = 22.4 L

0.441 moles = 22.4 * 0.441 = <u>9.88 L CO2 </u>

<u />

<u />

<u />

4.How many liters of water vapor would be produced in the combustion of 12.5L of ethane, C2H6 at STP?

2C2H6 + 7O2 →4CO2 + 6H2O

22.4 L = 1 mol

12.5 L = 0.848 moles C2H6

For 2 moles C2H6 we need 7 moles O2 to produce 4 moles CO2 and 6 moles H2O

For 0.848 moles C2H6 we'll have 3*0.848 =  2.544 moles H2O

1 mol = 22.4 L

2.544 moles = 22.4 L * 2.544 = <u>56.99 L H2O</u>

5 0
3 years ago
Other questions:
  • Is the equivilant of 39.3 inches​
    10·1 answer
  • What are three ways you might cause pollution
    10·1 answer
  • PLS ANSWER!! ASAP!! 10 POINTS!!
    9·1 answer
  • Consider the following reaction: CO(g)+2H2(g) &lt;--&gt; CH3OH(g).
    10·1 answer
  • The first eighteen chemical elements on the periodic table are described below in a series of statements. The first 1S letters o
    5·1 answer
  • Which of the following is a characteristic property of the noble gases?
    9·2 answers
  • What is the number of electrons and protons can be seen in this atom?
    15·1 answer
  • How do hospitals depend on oil?
    14·2 answers
  • Uifuiiiddiixkxkdkddkdi
    13·1 answer
  • Sterling silver is a mixture of the metals silver and copper.It is used to make jewellery.sometimes sterling silver causes green
    9·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!