Answer:
Check the explanation section.
Explanation:
The following steps should be followed orderly.
STEP ONE:
Use the dilution equation in the calculation of the volume for the stock solution. That is, C1 × V1 = C2 × V2.
Where C1 and C2 are the concentration of the stock solution and the diluted solution.
STEP TWO:
Put 6% of lactose and make sure to dilute it in order to make 2.0% lactose
and put it in Beaker A. Also, make sure to dilute the 1M to 0.1M SPG buffer in Beaker B.
STEP TWO:
Now, from beaker A containing 2% lactose, measure and remove 5.0 mL from it. Also, measure and remove 5.0 mL from beaker B containing 0.1M SPG.
So, in STEP TWO above we won't know how much water we need to use for dilution, thus, there is the need to make use of STEP ONE.
Therefore, from STEP ONE ABOVE, we have the dilution equation given as;
C1 × V1 = C2 × V2.
Hence, 6 × V1 = 2 × 5. Therefore, the volume needed from the stock solution, V1 = (2 × 5)/ 6 = 1.6 mL.
STEP THREE:
Now measure out 1.6 mL from the stock solution, that is 6% lactose and add it to 5mL of the diluted solution of 2% in beaker A into another container, say beaker C and add H2O to form SOLUTION X.
STEP FOUR:
Using the dilution equation again, Determine the the volume that is needed from 1M SPG.
C1 × V1 = C2 × V2.
V1 = ( 0.1 × 5)/ 1 = 0.5mL.
STEP FIVE:
measure 0.5mL out from the 1M SPG and 5 mL out of 0.1M SPG buffer and add water to it to form SOLUTION Y.
STEP SIX:
Now, mix solution X and solution Y together and take the required 5ml
