C ) prokaryotic - The cells that lack membrane bound organelles are called prokaryotic.
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
The odd one out would be the crow
During mitosis the cell splits and becomes two
Answer:
A DNA fragment with sticky end sequence TGGCA will bind with another DNA fragment with sticky end sequence ACCGT.
Explanation:
When a DNA strand is separated by the restriction endonuclease, it forms two separate single strands. These strands or cuts are known as sticky ends as they are detached from the complementary pairs.
These cuts of DNA are without complementary pairs and when they find suitable base pair, they get attached to it. These sticky ends are allowed to fix with the complementary base pairs during PCR/ polymerase chain reaction.
They are called sticky ends as they are ready to stick with the complementary base pairs of nucleotides.
