A locating agent is needed in an experiment to separate amino acids by chromatography as it helps to analyze colorless substances on paper.
<h3>What is chromatography?</h3>
Chromatography is an analytical method frequently used to separate a chemical mixture into its components, allowing for in-depth analysis of each component.
There are many different types of chromatography including liquid, gas, ion-exchange, and affinity chromatography, but they all use the same basic principle.
<h3>What is the use of a locating agent in Chromatography?</h3>
In paper chromatography, colorless compounds are examined using a locating agent.
It is a chemical that combines with colorless substances to produce colorful compounds that are easy to see for analysis. Ninhydrin spray is an example of a locating agent of this type.
Thus, a locating agent is used in an experiment to separate amino acids by chromatography as it helps in determining colorless substances on paper.
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Missing in your question :
Concentration by (M):
Xy: y: X
initial 0.2 0.3 0.3
change +X -X -X
equilibrim (0.2+x) (0.3-x) (0.3-x)
according to Kc formula: when Kc = 0.2
Kc = [XY]/[X]*[Y]
0.2 = (0.2+x) / (0.3-x)*(0.3-x)
0.2=(0.2+x) / (0.3-x)^2 by solving this equation
0.2*(0.3-x)^2 = 0.2+x
0.2* (0.09-0.6x+x^2)= 0.2 +x
0.0018 - 0.12 X +0.2X^2 = 0.2 + X
0.2X^2 -1.12 X -0.1982 = 0
∴X= 0.17
∴[XY] = 0.2 + 0.17 = 0.37 m
∴[X] = 0.3 - 0.17 =0.13 m
∴[y] = 0.3 - 0.17 = 0.13 m
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I believe the strength comes from its ability to break the particle bond in the molecule. So the bond dissociation energy