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3 years ago

Which enzyme is mixed with a DNA sample before gel electrophoresis

Biology

1 answer:

Svetach [21]3 years ago

4 0

Answer:

Restriction enzyme

Explanation:

Gel electrophoresis is a process or a technique in which fragments of DNA are separated in terms of their sizes.
The restriction enzyme is mixed with a DNA sample before the process. The restriction enzyme cut DNA at specific points into fragments which can then be separated based on their sizes on an agarose gel.
When current is applied to the gel DNA move towards the positively charged electrode since DNA is negatively charged, with shorter fragments migrating faster through the gel in comparison with large fragments.

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Which genotype determines that a person does NOT have sickle-cell anemia, but has the potential to pass the genetic disease on t

anyanavicka [17]

Answer:

The correct option is C) AS

Explanation:

Sickle cell anaemia is a recessive disorder in which the blood of the person is not able to clot properly. For sickle cell to occur, both the alleles for the trait have to be recessive. A person who has a dominant and a recessive allele will be heterozygous, showing the dominant characteristics. But such a person will be a carrier for the disease. There will be chances for the offsprings of that person to actually have the disease.

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3 years ago

Which statement most correctly describes the role of a connecting protein

Tcecarenko [31]

I remember the secondary structure by visualizing it. Understand that primary structure is just the order of the amino acids. The tertiary structure is the 3dimensional configuration of the whole protein molecule with multiple interactions guiding its fold (disulfide bonds, ionic interactions, hydrogen bonds, van der walls). Secondary structure is a step towards the conformation of the protein where it is made up of either alpha-helices or beta-pleated sheets. The alpha helices look like a spiraling staircase while the beta-pleated sheets look like a repetition of long loops. These two structures hold itself by hydrogen bonds.

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3 years ago

In a medicalseminar Dr. Rohith expressed his views on blood transfusion by saying "Hey friends during blood transfusion, any blo

Xelga [282]

Answer:

I agree and disagree with his statement.

Explanation:

We all know that the blood type AB is the universal recipient, so we know that this bloodtype can recieve any kind of blood. AB-type blood doesn't fight off anything because it has all of the antibodies needed. We also know that type O is the universal donor, meaning that this blood can be donated to anyone. This bloodtype doesn't have antibodies, so nothing will go wrong. However, for the other bloodtypes, yes, things have to be matched carefully. Type B blood can't be given type A blood, and so on. Another thing you have to think about is the Rh factor, which is the +/- after blood types. Rh+ bloodtypes can recieve both Rh+ and Rh- bloodtypes, but Rh- bloodtypes can only recieve Rh- bloodtypes. There are also graphic tables that will help answer this question if you're a visual learner!

I hope this helps, sorry it was a bit late!

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3 years ago

How do scientists know when life began?

Ronch [10]

Scientists so far haven’t managed to figure that out .

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3 years ago

Why can't both strands of DNA be replicated at the same time?

saw5 [17]

Answer: DNA is a molecule made up of two strands, twisted around each other in a double helix shape. The two strands are complementary which have a 5 prime end and a 3 prime end. To understand this question you must first understand the steps that follow.

DNA Replication:

Step one: 

DNA Helicase (unzips) separates the strands.

Step two:

DNA Primase starts the process and makes a small piece of RNA called a primer. This marks the starting point for the DNA.

Step three:

DNA Polymerase binds to the primer and will make the new strand of DNA. DNA Polymerase can only add DNA bases in one direction, from the 5 prime end to the 3 prime end.

The leading strand is made continuously.
The lagging strand does not run continuously because it runs in the opposite direction. Each fragment is started with an RNA primer. DNA Polymerase then adds a short row of DNA bases from the 5 prime to 3 prime direction. This results in okazaki fragments because it can only replicate in small chunks. The process is repeated.

Step four:

Once the new DNA is complete the enzyme exonuclease removes all the RNA primers from both strands of DNA.

Step five:

Another DNA Polymerase fills in the gaps that are left behind with DNA.

Step six:

DNA Ligase seals up the fragments in DNA, in both strands to make a continuous double strand.

Final answer:

DNA Replication cannot replicate at the same time due to the leading and lagging strand.

Good luck!

7 0

3 years ago