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A gene is a stretch of DNA or RNA that determines a certain trait. Genes mutate and can take two or more alternative forms; an allele is one of these forms of a gene. For example, the gene for eye color has several variations (alleles) such as an allele for blue eye color or an allele for brown eyes.
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Changes in the dna are called mutations. They can occur spontaneously. Mutations can only be passed down in gametes--sperm and egg cells. with Darwins theory at mind a mutation needs to pass the test of natural selection to remain in the gene pool. So purposly making mutation or altercations may end in diseases/illnesses or in some cases death because they body can not take it.....hope this helps if it doesnt im sorry!