The structure of this oligopeptide is attached:
It consists of arginine - alanine - phenylalanine and leucine
From the picture we can see that N terminus charge is +1
Also Arginine is considered as basic amino acid with charge +1
C terminus charge is -1
At pH = 8, the whose pKa are less than pH will be deprotonated and the net charge will be +1
So the net charge of this oligopeptide is +1 at pH = 8
Newton ,perhaps as many other originations use it as a gravitational law
I have never heard anyone ever say anything about aliens only living by using the same metabolistic processes as humans... But.. there may likely be another planet out there that does use oxygen, although at a different percentage from Earths. Anyways, even if we do find "other" life forms, who's to say that they're on the same level as us? Have you ever considered the fact that ants to us are like we are to other life forms out there and maybe they don't want or can't communicate with us because they see themselves as superior?
Hope that helps!
To make sure no cracks are in the crucible and also to remove any moisture present in the crucible by the process of heating.