Explanation:
For the detection of the antibody, several methods can be used:
Peroxidase is an enzyme that uses H2O2 to oxidize its substrates. These substrates are designed in such a way that their oxidation can be detected by light release (luminol), by the formation of a colored precipitate (DAB) or by the transformation of a colorless compound into a colorful compound, can be determined quantitatively by spectroscopy. Luminol, in the presence of H2O2 and peroxidase emits light, which can be captured by X-ray films, being mainly used in immunodetection. Diaminobanzidine (DAB), in the presence of H2O2 and peroxidase, forms a colored polymer brown, precipitating in the place where the peroxidase is found, being mainly used in immunocytochemistry studies. There is a wide variety of compounds that can be used as chromophores, providing the most varied colors, usable in ELISA technique, with ABTS (2, 2'-azino-di (3-ethylbenzthiazoline-6-sulfonate) being an example of dye that turns blue-green in the presence of peroxidase.
Alkaline phosphatase is an enzyme that hydrolyzes phosphate bonds. In this way, chromophores are compounds that have phosphate groups, and that with the loss of this group phosphates acquire color, as is the case with p-nitrophenyl phosphate, which turns yellow when phosphate group.
Fluorophore is a compound that emits light of a certain wavelength (λ (coloring) when excited by a light from another λ. Fluorophores with different characteristics make it possible to mark different antigens with different colors in the same tissue or cell, allowing simultaneous observation oftwo or more components.
- By Metals with high density
The main heavy metal conjugated to antibodies is gold. This allows you to locate antigens through electron microscopy, allowing very specific cytological studies
In psychology, frustration<span> is a common </span>emotional<span> response to opposition.
Related to </span>anger<span> and </span>disappointment<span>, frustration arises from the perceived resistance to the fulfilment of an individual's </span>will<span> or goal </span><span>and is likely to increase when a will or goal is denied or blocked.
There are two types of frustration; internal and external. Internal frustration may arise from challenges in fulfilling </span>personal goals<span>, </span>desires<span>, instinctual drives and needs, or dealing with perceived </span>deficiencies<span>, such as a lack of </span>confidence<span> or </span>fear<span> of social situations.
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Conflict<span>, such as when one has competing goals that interfere with one another, can also be an internal source of frustration and can create </span>cognitive dissonance<span>.
External causes of frustration involve conditions outside of an individual's control, such as a physical roadblock, a difficult task, or the perception of wasting time.
Hope that helped! :)</span>
Answer:
Rescheduling Thanksgiving dinner because of work.
Answer:
Transcendence
Explanation:
Transcendence -
It is the very last stage in the stages of change model .
In the stages of change model , it consists of six step , which is usually adapted in order to change any bad habit like , quitting smoking , drugs alcohol intake etc. , in order to attain a healthy lifestyle .
In this method , called the promised land .
The person no longer think about the bad habit and even do not require any distraction to quit the bad habit .
Hence , from the given scenario of the question ,
The correct answer is transcendence .
