Answer:
To determine the effect of toxin binding, you perform a Competitive Binding Experiment
Explanation:
Competitive binding experiments are very relevant when matters relating to cellular and or molecular toxicity are concerned.
They are used to determine the binding of a single concentration of labeled ligand in the presence of various concentrations of unlabeled ligand.
They are important because:
1. Validate a direct binding assay
2. Confirm whether or not a drug binds to the receptor
3. determine and explore how low-affinity drugs and receptors interact and it's resultant effects
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A plant cell is rectangular, while an animal cell is circular.
1. B
2. D
3. A
4. D
5. B
6. B
7. A
8. A
9. D
10. A
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Answer:
The provided events can be classified as:
Initiation
- In prokaryotes, the Shine-Dalgarno sequence pairs with rRNA.
- In E. coli, mRNA binds to the 30S ribosomal subunit.
Elongation
- In E. coli, EF-Tu delivers an aminoacyl-tRNA to the ribosome
- Initiator tRNA enters the P site.
- In E. coli, EF-Tu hydrolyzes.
- Translocation occurs
Termination
- The ribosome has mRNA, an empty A site, and deacylated tRNA in the P site
Translation is the process by which polypeptide chain is synthesized based on the codon sequence of the mRNA (messenger ribonucleic acid).
It is divided into three stages:
Initiation: The small and large sub-units of the ribosome get assembled around the initiation codon. It forms three sites (A, P, and E site) for interaction tRNA and mRNA.
Elongation: Specific charged-tRNA enters from A site and transfers its amino acid to the growing polypeptide chain in P site. Uncharged tRNA exit from the E site. Ribosome moves or translocates to the next codon.
Termination: As soon as ribosome reaches the termination codon, it releases the newly synthesized polypeptide chain.