i believe the answer is 300. ;)
Animals that have are invertebrate are generally insects, or very small animals, such as worms or snails.
Animals that have a vertebrate are generally larger animals,ranging from a small fish or bird, to a whale.
In short, a jaguar is a vertebrate.
Integumentary system I believe
Plasmid or vector transformation is the process by which exogenous DNA is transferred into the host cell. Transformation usually implies uptake of DNA into bacterial, yeast or plant cells, while transfection is a term usually reserved for mammalian cells.