In mammals and amphibians? An enucleated egg, a donor nucleus (preferably from an early developmental stage such as a blastocyst), and a means to stimulate the egg to be activated as if it had just been fertilized (poking with a needle is sometimes enough)
Or cloning into a vector as in at the level of DNA?
A vector with positve and negative selection markers (like antibiotic resistance and drug susceptibility), an insert, DNA ligase and restriction enzymes, buffer for restriction and ligation. Or if you are doing cloning by PCR, then you need primers, template, nucleotides, RNA pol like Taq polymerase etc.
Chemical reactions that release energy will not occur without a source of energy. So the answer is release.
Answer:
It will take 5492 seconds to electroplate 0.5 mm of gold on an object .
Explanation:
Mass of gold = m
Volume of gold = v
Surface area on which gold is plated = 
Thickness of the gold plating = h = 0.5 mm = 0.05 cm
1 mm = 0.1 cm

Density of the gold = 

Moles of gold = 

According to reaction, 1 mole of gold required 3 moles of electrons,then 0.152 moles of gold will require :
of electrons
Number of electrons = N =
Charge on single electron = 
Total charge required = Q

Amount of current passes = I = 8 Ampere
Duration of time = T



It will take 5492 seconds to electroplate 0.5 mm of gold on an object .