Answer:
A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.
B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.
On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.
Explanation:
Answer:
151.1J
Explanation:
Given parameters:
Mass of iron = 6.21g
Initial temperature of iron = 25°C
Final temperature of iron = 79.8°C
Unknown:
Amount of heat = ?
Solution:
The amount of heat require to cause this temperature can be determined using the expression below;
H = m c (T₂ - T₁)
H is the amount of heat
m is the mass
c is the specific heat capacity
T is the temperature
Specific heat capacity of iron 0.444J/g°C
Insert the parameters and solve;
H = 6.21 x 0.444 x (79.8 - 25)
H = 151.1J
Answer:
Oxygen.
Explanation:
They take in oxygen from the air. This is the process of respiration.
The Law of Conservation of Mass states that the mass of reactants entering a reaction must be equal to the mass of the products exiting it. In this case, we only have 2 reactants, Fe and S, and we only have 1 product, FeS. Therefore we expect the total mass of the Fe and S reactants to equal the mass of FeS. This gives us 112 g + 64 g = 176 g of FeS, which is choice D.
<u>Answer:</u> The new pressure will be 101.46 kPa.
<u>Explanation:</u>
To calculate the new pressure, we use the equation given by Gay-Lussac Law. This law states that pressure is directly proportional to the temperature of the gas at constant volume.
The equation given by this law is:
where,
are initial pressure and temperature.
are final pressure and temperature.
We are given:
By using conversion factor: 
Putting values in above equation, we get:
Hence, the new pressure will be 101.46 kPa.
