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Answer:
1. Percentage by weight = 0.5023 = 50.23 %
2. molar fraction =0.153
Explanation:
We know that
Molar mass of HClO4 = 100.46 g/mol
So the mass of 5 Moles= 5 x 100.46
Mass (m)= 5 x 100.46 = 502.3 g
Lets assume that aqueous solution of HClO4 and the density of solution is equal to density of water.
Given that concentration HClO4 is 5 M it means that it have 5 moles of HClO4 in 1000 ml.
We know that
Mass = density x volume
Mass of 1000 ml solution = 1 x 1000 =1000 ( density = 1 gm/ml)
m'=1000 g
1.
Percentage by weight = 502.3 /1000
Percentage by weight = 0.5023 = 50.23 %
2.
We know that
molar mass of water = 18 g/mol
mass of water in 1000 ml = 1000 - 502.3 g=497.9 g
So moles of water = 497.7 /18 mole
moles of water = 27.65 moles
So molar fraction = 5/(5+27.65)
molar fraction =0.153
Answer:
C. Trp D. Phe E. Tyr
Explanation:
The concentration of a protein has a direct relation with absorbance of the protein in a UV spectrophotometer. The formula which relates concentration with absorbance is described as under:
A = ∈ x c x l
where, A = Absorbance
∈ = Molar extinction co-efficient
c = Concentration of absorbing species i.e. protein
l = Path length of light
Tryptophan (Trp), phenylalanine (Phe ) and tyrosine (Tyr) are three aromatic amino acids which are used to measure protein concentration by UV. It is mainly because of tryptophan (Trp), protein absorbs at 280 nm which gives us an idea of protein concentration during UV spectroscopy.
The table depicting the wavelength at which these amino acids absorb and their respective molar extinction coefficient is as under:
Amino acid Wavelength Molar extinction co-efficient (∈)
Tryptophan 282 nm 5690
Tyrosine 274 nm 1280
Phenylalanine 257 nm 570
In view of table above, we can easily see that Molar extinction co-efficient (∈) of Tryptophan is highest amongst all these 3 amino acids that is why it dominates while measuring concentration.
Volume. Gases and liquids are typically measured in milliliters (mL) or cubic centimeters (cm^3) - both of which are equivalent (1 mL = 1 cm^3).
3.67 grams is the volume of the solution
