Location 2 is cooler than Location 3 and Location 1 because it's at a higher altitude
Abiotic factors are factors that include non-living parts of the environment which could be significant or essential to living organisms. Water is a non-living environment and it helps in giving life to plants and other organisms. Thus deemed abiotic.
ECM is the extracellular matrix, or the set of extracellular molecules secreted by cells with the purpose to support surrounding cells. In plants the cell wall is the ECM and in mammals ECM can be in the form of fibrils and may constitute a significant portion of the bulk of the organism and it also referred as connective tissue.
The answer is A. The ratio goes down as the size of the cell gets larger.