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3 years ago

8

Please tell me an example of a protein structure that would give positive test with Molisch’s Reagent?

2 answers:

Andru [333]3 years ago

7 0

Answer:

positive reaction for Molisch's test is given by almost all carbohydrates (exceptions include tetroses & trioses). It can be noted that even some glycoproteins and nucleic acids give positive results for this test (since they tend to undergo hydrolysis when exposed to strong mineral acids and form monosaccharides).

shtirl [24]3 years ago

7 0

Answer:

glycoproteins

Explanation:

A positive reaction for Molisch's test is given by almost all carbohydrates (exceptions include tetroses & trioses). It can be noted that even some glycoproteins and nucleic acids give positive results for this test (since they tend to undergo hydrolysis when exposed to strong mineral acids and form monosaccharides).

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Ganezh [65]

Answer:

pH = 2.03

Explanation:

The pH can be calculated using the following equation:

$pH = -log [H_{3}O^{+}]$ (1)

The concentration of H₃O⁺ is calculated using the dissociation constant of the next reaction:

CH₃COOH + H₂O ⇄ CH₃COO⁻ + H₃O⁺

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$K_{a} = \frac{[CH_{3}COO^{-}][H_{3}O^{+}]}{[CH_{3}COOH]}$

Solving the above equation for H₃O⁺, we have:

$[H_{3}O^{+}] = \frac{Ka*[CH_{3}COOH]}{[CH_{3}COO^{-}]}$ (2)

The dissociation constant is equal to:

$pKa = -log(Ka) \rightarrow Ka = 10^{-pKa} = 10^{-4.76} = 1.74 \cdot 10^{-5}$

Now, by solving the equation of the solubility product for Herbigon, we can find [CH₃COO⁻]:

CH₃COOX ⇄ CH₃COO⁻ + X⁺

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$K_{sp} = [CH_{3}COO^{-}][X^{+}]$

$[CH_{3}COO^{-}] = \frac{K_{sp}}{[X^{+}]} = \frac{9.40 \cdot 10^{-6}}{5.00 \cdot 10^{-3}} = 1.88 \cdot 10^{-3} M$

By entering the values of [CH₃COO⁻] and Ka, into equation (2) we can calculate [H₃O⁺]:

$[H_{3}O^{+}] = \frac{1.74 \cdot 10^{-5}*[1.00]}{[1.88 \cdot 10^{-3}]} = 9.26 \cdot 10^{-3} M$

Hence, the pH is:

$pH = -log [H_{3}O^{+}] = -log [9.26 \cdot 10^{-3}] = 2.03$

Therefore, the pH must be 2.03 to yield a solution in which the concentration of X⁺ is 5.00x10⁻³M.

I hope it helps you!

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