A bacterial promoter sequence must be included. The coding exons control the actual production of the protein that the gene codes for; meanwhile, the promoter sequence serves to initiate transcription of the specific genes. Promoters are included upstream of the DNA molecule at the start site of the gene.
It would be placer because you are not digging up any ground..if that is not right it might be none because if you are messing with water environments you are still messing with it which could not be good
C_____________________________________________________
c....ma'ma ik because im smart and if its not accurate then ill try again