Question

Expression vectors differ from cloning vectors by ________. the presence of a selectable marker gene the presence of sequences necessary to initiate replication the presence of necessary sequences to initiate transcription and translation a reduced number of cloning sites the ability to be introduced into host cells

Answer 1

Expression vectors differ from cloning vectors by the presence of necessary sequences to initiate transcription and translation. Expression vectors contain promoter sequences.

Expression vectors must contain all regulatory sequences to initiate transcription and translation.

Expression vectors contain:

• Promoter sequence
• Transcription termination sequence
• Ribosomal binding site (RBS)
• Operator (bacteria)

Examples of mammalian expression vectors include adenoviral vectors and retroviral vectors.

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