The Polymerase Chain Reaction is a technique widely used in molecular biology laboratories to amplify target DNA regions. The standard steps of a PCR are as follow 1-denaturation, 2-annealing and 3-elongation/extension. These steps are repeated 15-40 times in order to exponentially amplify the linear DNA fragment. It is well known that longer extension times can be used as a strategy to increase the yield of longer PCR products. This is because the extension time depends on the synthesis rate of the DNA polymerase used in PCR technique and the length of the DNA fragment to be amplified.

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2 years ago

Question 13 of 34

Gemiola [76]

Answer: A) Hydrogen Bond

Explanation:

A hydrogen bond occurs when a hydrogen (H) is covalently bound to a more electronegative atom or group forms an electrostatic attraction to another electronegative atom bearing a lone pair of electrons. This is possible due to the polarity of the H-electronegative atom bonds.