The answer is b just answerd this question in class<span />
If something isnt determined by look then that means you cant see it. Your answer is B) Knowledge about cars.
As the sodium borohydride and methanol responds with fluorenone it turns out to be more polar as it forms to fluorenol and so changes less on the TLC plate. Ever since TLC plates respond with polarity it can be observed the progress of the reaction.
It happens when the two groups become sufficiently different and the two can no longer interbreed. A known example of speciation is the London underground mosquito where it formed a new species within the existing genus. There are examples in micro-evolution but not in macro-evolution.