Question

Based on the passage, what is the structure of the product of the reaction between 8-hydroxyquinoline-5 sulfonate and hrp?.

Answer 1

A because the end result of this reaction is a radical created by the oxidation of an aromatic amine's or phenol's ring substituent. The hydroxyl group of a phenol acts as the ring substituent in this situation.

Which two enzyme types are required for the two-step process of converting cytosine to 5 hmC?

• The methyl group is transferred to cytosine in the first stage, and it is then hydroxylated in the second step.
• Therefore, a transferase and an oxidoreductase are the two groups of enzymes required.

Which kind of interaction between proteins and the dextran column material is most likely to take place?

• Hydrogen bonding because the glucose's OH would form an H-bond with any exposed polar side chains on a protein surface.

Two out of the four proteins would adhere to a cation-exchange column at what buffer pH?

• Only positively charged proteins can bind to a cation-exchange column, and this can only happen when the pH is lower than the pI.
• Proteins A and B would both be positively charged at pH 7.0.

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