Question

you will load a tlc plate with solution from each test tube of eluted solution. after visualizing your tlc plate, what would be the best possible outcome?

Answer 1

By loading the TLC plate from a solution with the eluted solution, the extraction of a required material after chromatography can be done through the eluted solution.

An eluted solution is generally used for the separation or extraction of one material from other through washing with the eluted solution.

By doing so, separate layers could be obtained. This process is usually done for extracting the pure form of RNA from cells, primary cell isolates, and animal and plant tissues from tumors.

Elution refers to the extraction of a substance from the solid adsorbed medium after the process of chromatography without impurities with the help of a solvent.

So, when a TLC plate is loaded from a solution to an eluted solution, then the adsorbed material from the solid medium gets extracted or separated. This would be the possible outcome.

To know more about chromatography, click below:

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