Question

how would the following changes influence the sensitivity of pcr primers for the target dna sequence in a pcr reaction? many mismatches between the target dna and primer sequences few mismatches between the target dna and primer sequences increase the annealing temperature decrease the extension temperature

Answer 1

The following changes influence the sensitivity of PCR primers for the target DNA sequence in a PCR reaction Increase.

The most effective way to improve specificity is to design primers that are nucleotides in length with a high annealing temperature of Tm. This is especially important when using genomic DNA or cDNA libraries as PCR templates. A pair of primers hybridizes with the sample DNA and defines the region to be amplified. The result is millions of copies in a very short time.

Primers are also used for DNA sequencing and other experimental procedures. A PCR primer refers to a short piece of single-stranded DNA used in a PCR reaction, and a sequencing primer refers to a short sequence of nucleotides used to initiate DNA synthesis in a sequencing reaction. Use the lowest possible concentration if necessary.

Learn more about A PCR reaction here:-brainly.com/question/15071698

#SPJ4