Before the development of electrophoresis to separate macromolecules, ____________ was used.

1 answer:

max2010maxim [7]1 year ago

4 0

Before the development of electrophoresis to separate macromolecules, high-speed centrifugation was used to isolate DNA.

A laboratory procedure called electrophoresis is used to divide DNA, RNA, or protein molecules according to their size and electrical charge. The molecules are moved by an electric current through a gel or other matrix. The technology of electrophoresis is crucial for the separation and examination of nucleic acids. At the lab bench, cloned DNA fragments are frequently isolated and worked with using nucleic acid electrophoresis.

High-speed centrifugation employs centrifugal force to separate particles with various densities or masses suspended in a liquid. High-speed rotation of the solution inside the tube causes each particle's angular momentum to experience centrifugal forces inversely proportionate to its mass.

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What is produced during the replacement reaction of ba(no3)2 and na2so4? 2bana 2no3so4 2nano3 baso4 nano3 baso4 bana2 (no3)2so4

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2NaNO3(aq) + BaSO4 = Ba(NO3)2(aq) + Na2SO4(aq) (s)

Procedures involved:

The cations or anions may transfer positions in this twofold replacement/displacement reaction, which results in AB + CD AD + CB. In such a reaction, water, an insoluble gas, or an insoluble solid must be one of the byproducts (precipitate). The reaction in question has the following molecular equation:

2NaNO3(aq) + BaSO4 = Ba(NO3)2(aq) + Na2SO4(aq) (s)

Double displacement:

When two atoms or groups of atoms swap positions, a double displacement reaction occurs, creating new compounds. Typically, aqueous solutions are where it happens.

Na2SO4 + BaCl2 BaSO4 + 2NaCl is an example of a double displacement reaction.

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Octanol is slightly soluble in water, and water is very soluble in octanol. why is it important to presaturate octanol with wate

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N-Octanol and water are chosen because the connection between a substance's hydrophilicity and lipophilicity is measured by $K_{OW}$ (n-Octanol/Water partition coefficient). When a chemical is more dissolves in fat-like solvents like n-octanol, the value is more significant than one, when it's more dissolved in water, the value is lower.

What is the partition coefficient?

The partition coefficient for the two-phase network comprising n-octanol and water is known as the $K_{OW}$ value. N-Octanol-Water Partition Ratio is another name for it.

The connection between a substance's hydrophilicity (its ability to dissolve in water) and lipophilicity (its ability to dissolve in fat) is measured by $K_{OW}$ . The value is bigger if a drug is more accessible in fat-like liquids like n-octanol and less if a compound seems more water-soluble.

Owing to linkage or fragmentation, substances that are involved in the octanol-water combination as multiple synthetic entities are each given a unique $K_{OW}$ ratio.

So, N-Octanol is chosen because it has a carbon/oxygen ratio that is comparable to that of lipids and because it shows both hydrophobic and hydrophilic properties. N-octanol, therefore, resembles the makeup and characteristics of cells and other living things.

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