Pyruvate is created in the cytoplasm via glycolysis, but it is oxidized in the mitochondrial matrix (in eukaryotes).
Pyruvate must enter the mitochondrion, penetrate its inner membrane, and reach the matrix before the chemical processes can commence.
The citric acid cycle occurs within the mitochondrion in the mitochondrial matrix, and oxidative metabolism happens at the internal folded mitochondrial membranes (cristae).
The citric acid cycle liberates stored energy by increasing acetyl-CoA oxidation. For eukaryotes, the process occurs within the mitochondrial matrix, while for prokaryotes, it occurs in the cytosol. The citric acid cycle generates three products: 1ATP, decreased FADH2 and NADH, and two molecules of CO2.
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