Question

A student isolates, purifies, and combines in a test tube a variety of molecules needed for DNA replication. After adding some DNA to the mixture, replication occurs, but each DNA molecule consists of a normal strand paired with numerous segments of DNA a few hundred nucleotides long. What has the student probably left out of the mixture

Answer 1

Answer:

DNA ligase

Explanation:

DNA replication is an enzyme mediated, 3-step process during which the DNA molecule produces a copy of itself. The 3 steps involved are as follows;

• initiation
• elongation
• termination

Initiation involves the unwinding of the double helix structure of the DNA using DNA helicase enzyme

Elongation involves binding of RNA primer to the DNA strands and addition of bases to the primer to elongate the new chains. Bases are added to the leading strand continuously while the lagging strand is replicated in short segments (okazaki segments).

Termination involves the unbinding of RNA primer and substitution of its bases by DNA bases. The Okazaki fragments are then joined together using the DNA ligase enzyme.

The short, numerous segments of DNA observed in the mixture is the Okazaki fragments of the lagging strand. This is due to the absence of the enzyme involved in the joining of these fragments.

Hence, the answer is DNA ligase.